Application | Comment | Organism |
---|---|---|
synthesis | - |
Geobacillus stearothermophilus |
synthesis | - |
Paenibacillus macerans |
synthesis | - |
Alkalihalobacillus alcalophilus |
synthesis | industrial strain E 192, industrial production of cyclodextrins | Niallia circulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
soluble starch | - |
Niallia circulans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Paenibacillus macerans | - |
- |
extracellular | strain E 192 | Niallia circulans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | protects enzyme from activity loss | Geobacillus stearothermophilus | |
Ca2+ | protects enzyme from activity loss | Niallia circulans | |
Ca2+ | protects enzyme from activity loss | Paenibacillus macerans | |
Ca2+ | protects enzyme from activity loss | Alkalihalobacillus alcalophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33500 | - |
E 192, gel filtration superose 12 column | Niallia circulans |
48500 | - |
E 192, Fractogel TSK HW 55 S | Niallia circulans |
78000 | - |
E 192, 2 isozymes, gel filtration, SDS-PAGE | Niallia circulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alkalihalobacillus alcalophilus | - |
- |
- |
Bacillus ohbensis | - |
- |
- |
Geobacillus stearothermophilus | - |
- |
- |
Klebsiella pneumoniae | - |
- |
- |
Micrococcus sp. | - |
- |
- |
Niallia circulans | - |
B. sp. var. alkalophilus No. 38-2, ATCC 21783 | - |
Niallia circulans | - |
no. 8 | - |
Niallia circulans | - |
E 192 | - |
Niallia circulans | - |
ATCC 9995 | - |
Niallia circulans 8 | - |
no. 8 | - |
Niallia circulans E 192 | - |
E 192 | - |
Paenibacillus macerans | - |
- |
- |
Priestia megaterium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
E 192 | Niallia circulans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
88 | - |
E 192, dextrinizing-like activity | Niallia circulans |
183.8 | - |
E 192, cyclodextrin synthesis | Niallia circulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-cyclodextrin + glycosyl acceptor | - |
Geobacillus stearothermophilus | beta-cyclodextrin + maltooligosaccharide | - |
r | |
alpha-cyclodextrin + glycosyl acceptor | - |
Niallia circulans | beta-cyclodextrin + maltooligosaccharide | - |
r | |
alpha-cyclodextrin + glycosyl acceptor | - |
Paenibacillus macerans | beta-cyclodextrin + maltooligosaccharide | - |
r | |
alpha-cyclodextrin + glycosyl acceptor | - |
Niallia circulans E 192 | beta-cyclodextrin + maltooligosaccharide | - |
r | |
alpha-cyclodextrin + glycosyl acceptor | - |
Niallia circulans 8 | beta-cyclodextrin + maltooligosaccharide | - |
r | |
Glucidex 12 + glycosyl acceptor | E 192 | Niallia circulans | beta-cyclodextrin | - |
r | |
Glucidex 2B + glycosyl acceptor | E 192 | Niallia circulans | beta-cyclodextrin | - |
r | |
Glucidex 2B + glycosyl acceptor | E 192 | Niallia circulans E 192 | beta-cyclodextrin | - |
r | |
Glucidex 2B + glycosyl acceptor | E 192 | Niallia circulans 8 | beta-cyclodextrin | - |
r | |
glycogen + acceptor | - |
Geobacillus stearothermophilus | beta-cyclodextrin | - |
? | |
glycogen + acceptor | - |
Paenibacillus macerans | beta-cyclodextrin | - |
r | |
glycogen + acceptor | E 192 | Niallia circulans | beta-cyclodextrin | - |
r | |
maltoheptaose + glycosyl acceptor | E 192 | Niallia circulans | beta-cyclodextrin | - |
r | |
maltohexaose + glycosyl acceptor | E 192 | Niallia circulans | beta-cyclodextrin | - |
r | |
maltose + glycosyl acceptor | - |
Geobacillus stearothermophilus | beta-cyclodextrin | - |
r | |
maltose + glycosyl acceptor | E 192, poor substrate | Niallia circulans | beta-cyclodextrin | - |
r | |
maltotriose + glycosyl acceptor | - |
Geobacillus stearothermophilus | beta-cyclodextrin | - |
r | |
maltotriose + glycosyl acceptor | E 192 | Niallia circulans | beta-cyclodextrin | - |
r | |
additional information | - |
Geobacillus stearothermophilus | ? | - |
? | |
additional information | - |
Paenibacillus macerans | ? | - |
? | |
additional information | - |
Alkalihalobacillus alcalophilus | ? | - |
? | |
additional information | glucose is no substrate | Niallia circulans | ? | - |
? | |
additional information | glucose is no substrate | Niallia circulans E 192 | ? | - |
? | |
additional information | glucose is no substrate | Niallia circulans 8 | ? | - |
? | |
soluble starch + glycosyl acceptor | - |
Geobacillus stearothermophilus | cyclodextrins | - |
r | |
soluble starch + glycosyl acceptor | - |
Niallia circulans | cyclodextrins | - |
r | |
soluble starch + glycosyl acceptor | - |
Paenibacillus macerans | cyclodextrins | - |
r | |
soluble starch + glycosyl acceptor | - |
Micrococcus sp. | cyclodextrins | - |
r | |
soluble starch + glycosyl acceptor | - |
Niallia circulans E 192 | cyclodextrins | - |
r | |
soluble starch + glycosyl acceptor | - |
Niallia circulans 8 | cyclodextrins | - |
r | |
soluble starch + glycosyl acceptor | - |
Priestia megaterium | beta-cyclodextrin | - |
r | |
soluble starch + glycosyl acceptor | - |
Paenibacillus macerans | beta-cyclodextrin | - |
? | |
soluble starch + glycosyl acceptor | - |
Bacillus ohbensis | beta-cyclodextrin | - |
r | |
soluble starch + glycosyl acceptor | E 192, waxy maize starch is the best substrate, wheat starch, corn starch, potato starch | Niallia circulans | beta-cyclodextrin | - |
r | |
soluble starch + glycosyl acceptor | E 192, waxy maize starch is the best substrate, wheat starch, corn starch, potato starch | Niallia circulans E 192 | beta-cyclodextrin | - |
r | |
soluble starch + glycosyl acceptor | E 192, waxy maize starch is the best substrate, wheat starch, corn starch, potato starch | Niallia circulans 8 | beta-cyclodextrin | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Paenibacillus macerans |
monomer | 1 * 78000, E 192, SDS-PAGE | Niallia circulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Geobacillus stearothermophilus |
60 | - |
- |
Paenibacillus macerans |
60 | - |
- |
Bacillus ohbensis |
60 | - |
E 192 | Niallia circulans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | strain E 192, heat labile, rapid inactivation at temperatures above 45, remaining activity is 75% after 20 min at 40°C, 10% at 50°C and only 5% after 1 min at 60°C, protected by substrate and Ca2+ enzyme is stable for at least 24 h at pH 6.0, very stable at pH 7.0, 48 h without any loss of activity | Niallia circulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Geobacillus stearothermophilus |
5.5 | - |
- |
Niallia circulans |
5.5 | - |
- |
Paenibacillus macerans |
5.5 | - |
- |
Bacillus ohbensis |
5.5 | 5.8 | strain E 192 | Niallia circulans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | strain E 192, only 14% decrease in activity | Niallia circulans |
7 | 8 | strain E 192, maximum stability, only 14% decrease in activity at pH 7.0 | Niallia circulans |