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Literature summary for 2.4.1.19 extracted from

  • Bovetto, L.J.; Backer, D.P.; Villette, J.R.; Sicard, P.J.; Bouquelet, S.J.L.
    Cyclomaltodextrin glucanotransferase from Bacillus circulans E 192. I. Purification and characterization of the enzyme (1992), Biotechnol. Appl. Biochem., 15, 48-58.
    View publication on PubMed

Application

Application Comment Organism
synthesis
-
Geobacillus stearothermophilus
synthesis
-
Paenibacillus macerans
synthesis
-
Alkalihalobacillus alcalophilus
synthesis industrial strain E 192, industrial production of cyclodextrins Niallia circulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
soluble starch
-
Niallia circulans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Paenibacillus macerans
-
-
extracellular strain E 192 Niallia circulans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ protects enzyme from activity loss Geobacillus stearothermophilus
Ca2+ protects enzyme from activity loss Niallia circulans
Ca2+ protects enzyme from activity loss Paenibacillus macerans
Ca2+ protects enzyme from activity loss Alkalihalobacillus alcalophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33500
-
E 192, gel filtration superose 12 column Niallia circulans
48500
-
E 192, Fractogel TSK HW 55 S Niallia circulans
78000
-
E 192, 2 isozymes, gel filtration, SDS-PAGE Niallia circulans

Organism

Organism UniProt Comment Textmining
Alkalihalobacillus alcalophilus
-
-
-
Bacillus ohbensis
-
-
-
Geobacillus stearothermophilus
-
-
-
Klebsiella pneumoniae
-
-
-
Micrococcus sp.
-
-
-
Niallia circulans
-
B. sp. var. alkalophilus No. 38-2, ATCC 21783
-
Niallia circulans
-
no. 8
-
Niallia circulans
-
E 192
-
Niallia circulans
-
ATCC 9995
-
Niallia circulans 8
-
no. 8
-
Niallia circulans E 192
-
E 192
-
Paenibacillus macerans
-
-
-
Priestia megaterium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
E 192 Niallia circulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
88
-
E 192, dextrinizing-like activity Niallia circulans
183.8
-
E 192, cyclodextrin synthesis Niallia circulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-cyclodextrin + glycosyl acceptor
-
Geobacillus stearothermophilus beta-cyclodextrin + maltooligosaccharide
-
r
alpha-cyclodextrin + glycosyl acceptor
-
Niallia circulans beta-cyclodextrin + maltooligosaccharide
-
r
alpha-cyclodextrin + glycosyl acceptor
-
Paenibacillus macerans beta-cyclodextrin + maltooligosaccharide
-
r
alpha-cyclodextrin + glycosyl acceptor
-
Niallia circulans E 192 beta-cyclodextrin + maltooligosaccharide
-
r
alpha-cyclodextrin + glycosyl acceptor
-
Niallia circulans 8 beta-cyclodextrin + maltooligosaccharide
-
r
Glucidex 12 + glycosyl acceptor E 192 Niallia circulans beta-cyclodextrin
-
r
Glucidex 2B + glycosyl acceptor E 192 Niallia circulans beta-cyclodextrin
-
r
Glucidex 2B + glycosyl acceptor E 192 Niallia circulans E 192 beta-cyclodextrin
-
r
Glucidex 2B + glycosyl acceptor E 192 Niallia circulans 8 beta-cyclodextrin
-
r
glycogen + acceptor
-
Geobacillus stearothermophilus beta-cyclodextrin
-
?
glycogen + acceptor
-
Paenibacillus macerans beta-cyclodextrin
-
r
glycogen + acceptor E 192 Niallia circulans beta-cyclodextrin
-
r
maltoheptaose + glycosyl acceptor E 192 Niallia circulans beta-cyclodextrin
-
r
maltohexaose + glycosyl acceptor E 192 Niallia circulans beta-cyclodextrin
-
r
maltose + glycosyl acceptor
-
Geobacillus stearothermophilus beta-cyclodextrin
-
r
maltose + glycosyl acceptor E 192, poor substrate Niallia circulans beta-cyclodextrin
-
r
maltotriose + glycosyl acceptor
-
Geobacillus stearothermophilus beta-cyclodextrin
-
r
maltotriose + glycosyl acceptor E 192 Niallia circulans beta-cyclodextrin
-
r
additional information
-
Geobacillus stearothermophilus ?
-
?
additional information
-
Paenibacillus macerans ?
-
?
additional information
-
Alkalihalobacillus alcalophilus ?
-
?
additional information glucose is no substrate Niallia circulans ?
-
?
additional information glucose is no substrate Niallia circulans E 192 ?
-
?
additional information glucose is no substrate Niallia circulans 8 ?
-
?
soluble starch + glycosyl acceptor
-
Geobacillus stearothermophilus cyclodextrins
-
r
soluble starch + glycosyl acceptor
-
Niallia circulans cyclodextrins
-
r
soluble starch + glycosyl acceptor
-
Paenibacillus macerans cyclodextrins
-
r
soluble starch + glycosyl acceptor
-
Micrococcus sp. cyclodextrins
-
r
soluble starch + glycosyl acceptor
-
Niallia circulans E 192 cyclodextrins
-
r
soluble starch + glycosyl acceptor
-
Niallia circulans 8 cyclodextrins
-
r
soluble starch + glycosyl acceptor
-
Priestia megaterium beta-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Paenibacillus macerans beta-cyclodextrin
-
?
soluble starch + glycosyl acceptor
-
Bacillus ohbensis beta-cyclodextrin
-
r
soluble starch + glycosyl acceptor E 192, waxy maize starch is the best substrate, wheat starch, corn starch, potato starch Niallia circulans beta-cyclodextrin
-
r
soluble starch + glycosyl acceptor E 192, waxy maize starch is the best substrate, wheat starch, corn starch, potato starch Niallia circulans E 192 beta-cyclodextrin
-
r
soluble starch + glycosyl acceptor E 192, waxy maize starch is the best substrate, wheat starch, corn starch, potato starch Niallia circulans 8 beta-cyclodextrin
-
r

Subunits

Subunits Comment Organism
monomer
-
Paenibacillus macerans
monomer 1 * 78000, E 192, SDS-PAGE Niallia circulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Geobacillus stearothermophilus
60
-
-
Paenibacillus macerans
60
-
-
Bacillus ohbensis
60
-
E 192 Niallia circulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 strain E 192, heat labile, rapid inactivation at temperatures above 45, remaining activity is 75% after 20 min at 40°C, 10% at 50°C and only 5% after 1 min at 60°C, protected by substrate and Ca2+ enzyme is stable for at least 24 h at pH 6.0, very stable at pH 7.0, 48 h without any loss of activity Niallia circulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Geobacillus stearothermophilus
5.5
-
-
Niallia circulans
5.5
-
-
Paenibacillus macerans
5.5
-
-
Bacillus ohbensis
5.5 5.8 strain E 192 Niallia circulans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 strain E 192, only 14% decrease in activity Niallia circulans
7 8 strain E 192, maximum stability, only 14% decrease in activity at pH 7.0 Niallia circulans