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Literature summary for 2.4.1.19 extracted from
- Immobilzed cyclomaltodextrin glucanotransferase of an alkalophilic Bacillus sp. No. 38-2 (1984), Biotechnol. Bioeng., 26, 595-598.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Geobacillus stearothermophilus |  |
| Ca2+ | - |
Bacillus sp. (in: Bacteria) | |
| Ca2+ | - |
Paenibacillus macerans | |
| Ca2+ | addition increases thermal stability | Niallia circulans | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
- |
- |
| Geobacillus stearothermophilus | - |
- |
- |
| Klebsiella pneumoniae | - |
- |
- |
| Niallia circulans | - |
B. sp. var. alkalophilus No. 38-2, ATCC 21783 | - |
| Paenibacillus macerans | - |
- |
- |
| Priestia megaterium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ATCC 21783 | Niallia circulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + glycosyl acceptor | ATCC 21783 | Niallia circulans | cycloheptaamylose + cyclohexaamylose + exo-branched cyclohexaamylose | - |
r | |
| amylose + glycosyl acceptor | - |
Geobacillus stearothermophilus | ? | - |
r | |
| amylose + glycosyl acceptor | ATCC 21783 | Niallia circulans | ? | - |
r | |
| glycogen + acceptor | - |
Geobacillus stearothermophilus | beta-cyclodextrin | - |
? | |
| glycogen + acceptor | - |
Paenibacillus macerans | beta-cyclodextrin | - |
r | |
| glycogen + acceptor | ATCC 21783 | Niallia circulans | beta-cyclodextrin | - |
r | |
| maltooligosaccharides + glycosyl acceptor | - |
Geobacillus stearothermophilus | cyclodextrins | - |
r | |
| maltooligosaccharides + glycosyl acceptor | - |
Paenibacillus macerans | cyclodextrins | - |
r | |
| maltooligosaccharides + glycosyl acceptor | ATCC 21783 | Niallia circulans | cyclodextrins | - |
r | |
| additional information | - |
Geobacillus stearothermophilus | ? | - |
? | |
| additional information | - |
Paenibacillus macerans | ? | - |
? | |
| additional information | ATCC 21783 contains 3 types of enzymes, acid, neutral and alcaline | Niallia circulans | ? | - |
? | |
| soluble starch + glycosyl acceptor | - |
Geobacillus stearothermophilus | cyclodextrins | - |
r | |
| soluble starch + glycosyl acceptor | - |
Niallia circulans | cyclodextrins | - |
r | |
| soluble starch + glycosyl acceptor | - |
Paenibacillus macerans | cyclodextrins | - |
r | |
| soluble starch + glycosyl acceptor | - |
Geobacillus stearothermophilus | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Priestia megaterium | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Bacillus sp. (in: Bacteria) | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Paenibacillus macerans | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | ATCC 21783 | Niallia circulans | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus sp. (in: Bacteria) |
| 50 | - |
ATCC 21783 | Niallia circulans |
| 55 | - |
- |
Geobacillus stearothermophilus |
| 55 | - |
- |
Priestia megaterium |
| 55 | - |
- |
Bacillus sp. (in: Bacteria) |
| 55 | - |
- |
Paenibacillus macerans |
| 55 | - |
ATCC 21783, immobilized enzyme | Niallia circulans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | - |
Paenibacillus macerans |
| 30 | 70 | ATCC 21783 | Niallia circulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
ATCC 21783, stable at pH 8.0 for about 2 weeks | Niallia circulans |
| 65 | - |
ATCC 21783, stable up to | Niallia circulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
ATCC 21783 contains 3 isozymes, neutral, alkalic and acidic, possessing markedly different pH optima, 4.6, 7.0 and 9.5 | Niallia circulans |
| 9 | - |
- |
Bacillus sp. (in: Bacteria) |
| 9 | - |
ATCC 21783, immobilized enzyme, acid enzyme activity disappeared | Niallia circulans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | ATCC 21783 | Niallia circulans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | ATCC 21783, immobilized enzyme, stable | Niallia circulans |
| 8 | - |
ATCC 21783, stable for about 2 weeks at 60°C | Niallia circulans |
| 10 | 11 | ATCC 21783, stability decreases by immobilization | Niallia circulans |
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