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Literature summary for 2.4.1.18 extracted from
- The unique branching patterns of Deinococcus glycogen branching enzymes are determined by their N-terminal domains (2009), Appl. Environ. Microbiol., 75, 1355-1362.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 | Deinococcus geothermalis |
| expression in Escherichia coli BL21 | Deinococcus radiodurans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | CT Dg, truncated at 3' end | Deinococcus geothermalis |
| additional information | CTT Dr, tuncated at 3' end | Deinococcus radiodurans |
| additional information | GGR, construction of chimeric genes, with C-domain of GBE of Deinococcus radiodurans | Deinococcus geothermalis |
| additional information | GGR, construction of chimeric genes, with N-domain and A-domain of GBE of Deinococcus geothermalis | Deinococcus radiodurans |
| additional information | GRR, construction of chimeric genes, with A-domain and C-domain of GBE of Deinococcus radiodurans | Deinococcus geothermalis |
| additional information | GRR, construction of chimeric genes, with N-domain of GBE of Deinococcus geothermalis | Deinococcus radiodurans |
| additional information | NT Dg, tuncated at 5' end | Deinococcus geothermalis |
| additional information | RGG, construction of chimeric genes, with A-domain and C-domain of GBE of Deinococcus geothermalis | Deinococcus radiodurans |
| additional information | RGG, construction of chimeric genes, with N-domain of GBE of Deinococcus radiodurans | Deinococcus geothermalis |
| additional information | RRG, construction of chimeric genes, with C-domain of GBE of Deinococcus geothermalis | Deinococcus radiodurans |
| additional information | RRG, construction of chimeric genes, with N-domain and A-domain of GBE of Deinococcus radiodurans | Deinococcus geothermalis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Deinococcus geothermalis | - |
- |
| soluble | - |
Deinococcus radiodurans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
SDS-PAGE | Deinococcus geothermalis |
| 80000 | - |
SDS-PAGE | Deinococcus radiodurans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Deinococcus geothermalis | Q1IZQ3 | strain DSM 11300 | - |
| Deinococcus radiodurans | Q9RTB7 | - |
- |
| Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 | Q9RTB7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His tag affinity chromatography | Deinococcus geothermalis |
| His tag affinity chromatography | Deinococcus radiodurans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activity on amylopectin 342.3 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant RGG, iodine assay | Deinococcus geothermalis |
| additional information | - |
specific activity on amylopectin 342.3 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant RGG, iodine assay | Deinococcus radiodurans |
| additional information | - |
specific activity on amylopectin 359.2 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant GGR, iodine assay | Deinococcus geothermalis |
| additional information | - |
specific activity on amylopectin 359.2 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant GGR, iodine assay | Deinococcus radiodurans |
| additional information | - |
specific activity on amylopectin 474.3 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, wild-type enzyme, iodine assay | Deinococcus geothermalis |
| additional information | - |
specific activity on amylopectin 505.6 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant RRG, iodine assay | Deinococcus geothermalis |
| additional information | - |
specific activity on amylopectin 505.6 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant RRG, iodine assay | Deinococcus radiodurans |
| additional information | - |
specific activity on amylopectin 538.0 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, wild-type enzyme, iodine assay | Deinococcus radiodurans |
| additional information | - |
specific activity on amylopectin 556.5 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant CTT Dr, iodine assay | Deinococcus radiodurans |
| additional information | - |
specific activity on amylopectin less than 0.5 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant GRR, iodine assay | Deinococcus geothermalis |
| additional information | - |
specific activity on amylopectin less than 0.5 U/mg, 1 U = decrease in absorbance of 1.0 per min at 530 nm, mutant GRR, iodine assay | Deinococcus radiodurans |
| additional information | - |
specific activity on amylose 404.2 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, wild-type enzyme, iodine assay | Deinococcus radiodurans |
| additional information | - |
specific activity on amylose 411.7 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant RRG, iodine assay | Deinococcus geothermalis |
| additional information | - |
specific activity on amylose 411.7 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant RRG, iodine assay | Deinococcus radiodurans |
| additional information | - |
specific activity on amylose 442.3 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant CTT Dr, iodine assay | Deinococcus radiodurans |
| additional information | - |
specific activity on amylose 468.8 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant GGR, iodine assay | Deinococcus geothermalis |
| additional information | - |
specific activity on amylose 468.8 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant GGR, iodine assay | Deinococcus radiodurans |
| additional information | - |
specific activity on amylose 621.3 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, wild-type enzyme, iodine assay | Deinococcus geothermalis |
| additional information | - |
specific activity on amylose 624.1 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant RGG, iodine assay | Deinococcus geothermalis |
| additional information | - |
specific activity on amylose 624.1 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant RGG, iodine assay | Deinococcus radiodurans |
| additional information | - |
specific activity on amylose less than 0.5 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant GRR, iodine assay | Deinococcus geothermalis |
| additional information | - |
specific activity on amylose less than 0.5 U/mg, 1 U = decrease in absorbance of 1.0 per min at 660 nm, mutant GRR, iodine assay | Deinococcus radiodurans |
| 4.7 | - |
wild-type enzyme, branching assay | Deinococcus radiodurans |
| 7.2 | - |
wild-type enzyme, branching assay | Deinococcus geothermalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin | iodine assay | Deinococcus geothermalis | ? | - |
? |  |
| amylopectin | iodine assay | Deinococcus radiodurans | ? | - |
? | |
| amylopectin | iodine assay | Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 | ? | - |
? | |
| amylose | branching assay, at pH 8.0, reaction stopped by boiling for 5 min | Deinococcus geothermalis | ? | - |
? | |
| amylose | branching assay, at pH 8.0, reaction stopped by boiling for 5 min | Deinococcus radiodurans | ? | - |
? | |
| amylose | branching assay, at pH 8.0, reaction stopped by boiling for 5 min | Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1,4-alpha-glucan branching enzyme | - |
Deinococcus geothermalis |
| 1,4-alpha-glucan branching enzyme | - |
Deinococcus radiodurans |
| Dg GBE | - |
Deinococcus geothermalis |
| Dr GBE | - |
Deinococcus radiodurans |
| GH13 GBE | - |
Deinococcus geothermalis |
| GH13 GBE | - |
Deinococcus radiodurans |
| glycogen branching enzyme | - |
Deinococcus geothermalis |
| glycogen branching enzyme | - |
Deinococcus radiodurans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 34 | - |
iodine assay | Deinococcus geothermalis |
| 34 | - |
iodine assay | Deinococcus radiodurans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | iodine assay | Deinococcus geothermalis |
| 30 | 40 | iodine assay | Deinococcus radiodurans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
after 1 h incubation at fully active | Deinococcus radiodurans |
| 60 | 65 | after 1 h incubation at 60°C fully active, after 1 h incubation at 65°C only 80% residual activity | Deinococcus geothermalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
iodine assay | Deinococcus geothermalis |
| 8 | - |
iodine assay | Deinococcus radiodurans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | iodine assay | Deinococcus geothermalis |
| 7 | 9 | iodine assay | Deinococcus radiodurans |
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Release 2023.1 (January 2023)
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