Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, full-length enzyme and a truncated enzyme form missing the first 107 amino acids | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop method, active N-terminally truncated form missing the first 107 amino acids | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
truncated enzyme form missing the first 107 amino- | the purified full-length enzyme is poorly soluble and forms aggregates, which are inactive, at concentrations above 1 mg/ml. In contrast, the truncated form can be concentrated to 6 mg/ml without a visible signs of aggregation or loss of activity on concentration | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | the purified full-length enzyme is poorly soluble and forms aggregates, which are inactive, at concentrations above 1 mg/ml. In contrast, the truncated form can be concentrated to 6 mg/ml without a visible sign of aggregation or loss of activity on concentration | Escherichia coli | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | the enzyme is responsible for the formation of the alpha-1,6 linkages in the glycogen molecule | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is responsible for the formation of the alpha-1,6 linkages in the glycogen molecule | Escherichia coli | ? | - |
? |