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Literature summary for 2.4.1.17 extracted from

  • Basu, N.K.; Kovarova, M.; Garza, A.; Kubota, S.; Saha, T.; Mitra, P.S.; Banerjee, R.; Rivera, J.; Owens, I.S.
    Phosphorylation of a UDP-glucuronosyltransferase regulates substrate specificity (2005), Proc. Natl. Acad. Sci. USA, 102, 6285-6290.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Cos-1 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
S432G mutant enzyme retains full activity, shift in pH-optimum for glucuronidation of eugenol from pH 6.0 for wild-type enzyme to 6.4 for mutant enzyme S432G Homo sapiens
S432G shift in pH-optimum for glucuronidation of eugenol from pH 8.5 for wild-type enzyme to 6.4 for mutant enzyme S432G. S432G-UGT1A7, but not wild-type UGT1A7, metabolizes new substrates, 17beta-estradiol, flurbiprofen, and estriol Homo sapiens
T202A inactive mutant enzyme Homo sapiens
T73A inactive mutant enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9HAW7
-
-
Homo sapiens Q9HAW8
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein phosphorylation of a UDP-glucuronosyltransferase by protein kinase C regulates substrate specificity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17beta-estradiol + UDP-glucuronate metabilized by mutant enzyme S432G-UGT1A7, but not by wild-type enzyme UGT1A7 Homo sapiens ?
-
?
estriol + UDP-glucuronate metabilized by mutant enzyme S432G-UGT1A7, but not by wild-type enzyme UGT1A7 Homo sapiens ?
-
?
eugenol + UDP-glucuronate
-
Homo sapiens ?
-
?
eugenol + UDP-glucuronate
-
Homo sapiens UDP + eugenol-O-alpha-D-glucuronide
-
?
flurbiprofen + UDP-glucuronate metabilized by mutant enzyme S432G-UGT1A7, but not by wild-type enzyme UGT1A7 Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
UGT1A10
-
Homo sapiens
UGT1A7
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
substrate: eugenol, wild-type enzyme Homo sapiens
6.4
-
substrate: eugenol, mutant enzyme S432G Homo sapiens
8.5
-
substrate: eugenol, wild-type enzyme Homo sapiens