Literature summary for 2.4.1.17 extracted from

Barre, L.; Fournel-Gigleux, S.; Finel, M.; Netter, P.; Magdalou, J.; Ouzzine, M.
Substrate specificity of the human UDP-glucuronosyltransferase UGT2B4 and UGT2B7. Identification of a critical aromatic amino acid residue at position 33 (2007), FEBS J., 274, 1256-1264.

Search for more literature for 2.4.1.17

Cloned(Commentary)

Cloned (Comment)	Organism
heterologous expression in insect Sf9 cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
F33L	mutation suppresses the activity towards hyodeoxycholic acid, and impairs the glucuronidation of several substrates, including 4-hydroxyestrone and 17-epiestriol	Homo sapiens
F33Y	activity is similar to wild-type UGT2B4. Tyrosine is found at position 33 of UGT2B7	Homo sapiens
Y33F	mutation exhibits similar or even somewhat higher activities than wild type UGT2B7. Phenylalanine is found at position 33 of UGT2B4	Homo sapiens
Y33L	mutation strongly reduces the activity towards all the tested substrates, with the exception of 17-epiestriol	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.012	-	montanoyl-CoA	mutant UGT2B4(Y33L)	Homo sapiens
0.021	-	hyodeoxycholic acid	native UGT2B4	Homo sapiens
0.025	-	hyodeoxycholic acid	native UGT2B4	Homo sapiens
0.028	-	MOCAc-Lys-Arg-Gly-Leu-Tyr-Phe-Ile-Thr-His-Lys(Dnp)	native UGT2B4	Homo sapiens
0.029	-	hyodeoxycholic acid	mutant UGT2B4(Y33L)	Homo sapiens
0.039	-	hyodeoxycholic acid	mutant UGT2B4(Y33F)	Homo sapiens
0.042	-	17-epiestriol	native UGT2B4	Homo sapiens
0.052	-	17-epiestriol	native UGT2B4	Homo sapiens
0.063	-	17-epiestriol	mutant UGT2B4(Y33L)	Homo sapiens
0.071	-	17-epiestriol	mutant UGT2B4(F33Y)	Homo sapiens
0.081	-	montanoyl-CoA	native UGT2B4	Homo sapiens
0.091	-	hyodeoxycholic acid	mutant UGT2B4(F33Y)	Homo sapiens
0.093	-	17-epiestriol	mutant UGT2B4(F33L)	Homo sapiens
0.117	-	MOCAc-Lys-Arg-Gly-Leu-Tyr-Phe-Ile-Thr-His-Lys(Dnp)	mutant UGT2B4(Y33F)	Homo sapiens
0.131	-	MOCAc-Lys-Arg-Gly-Leu-Tyr-Phe-Ile-Thr-His-Lys(Dnp)	mutant UGT2B4(F33Y)	Homo sapiens
0.159	-	17-epiestriol	mutant UGT2B4(Y33F)	Homo sapiens
0.173	-	MOCAc-Lys-Arg-Gly-Leu-Tyr-Phe-Ile-Thr-His-Lys(Dnp)	mutant UGT2B4(F33L)	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P06133	-	-
Homo sapiens	P16662	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
17-epiestriol + UDP-glucuronate	-	Homo sapiens	?	-	?
4-hydroxyestrone + UDP-glucuronate	-	Homo sapiens	?	-	?
hyodeoxycholic acid + UDP-glucuronate	-	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
UGT2B4	-	Homo sapiens
UGT2B7	-	Homo sapiens

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Release 2023.1 (January 2023)