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Literature summary for 2.4.1.17 extracted from

  • Matern, H.; Matern, S.; Schelzig, C.; Gerok, W.
    Bile acid UDP-glucoronyltransferase from human liver. Properties and studies on aglycone substrate specificity (1980), FEBS Lett., 118, 251-254.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Clofibrate
-
Homo sapiens
Digitonin 0.6 mg/mg liver protein, 1.8fold activation using non-saturated substrate concentration Homo sapiens
Triton X-100 0.01%, 1.6fold activation using non-saturated substrate concentration Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-nitrophenol uncompetitive to chenodeoxycholic acid Homo sapiens
bilirubin competitive to chenodeoxycholic acid Homo sapiens
chenodeoxycholic acid inhibits glucuronidation of bilirubin Homo sapiens
Digitonin inhibition above 0.6 mg/mg protein; no effect at saturating substrate concentrations Homo sapiens
estradiol competitive to chenodeoxycholic acid Homo sapiens
Fe2+
-
Homo sapiens
Ni2+
-
Homo sapiens
testosterone noncompetitive to chenodeoxycholic acid Homo sapiens
Triton X-100 above 0.01% v/v; no effect at saturating substrate concentrations Homo sapiens
Zn2+
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.083
-
chenodeoxycholic acid
-
Homo sapiens
0.96
-
UDPglucuronic acid
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 140% stimulated by 5mM Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chenodeoxycholic acid + UDP-glucuronate
-
Homo sapiens UDP + ?
-
?
cholic acid + UDPglucuronate
-
Homo sapiens cholic acid O-glucuronide
-
?
lithocholic acid + UDPglucuronate
-
Homo sapiens UDP + ?
-
?
additional information 36 substrates for HGT2B7H and HGT2B7Y. HGT2B7 glucuronidates both hydroxyl and carboxyl functions of bile acids, retinols and hydroxylated fatty acids Homo sapiens ?
-
?
additional information affinity of unconjugated bile acids decrease with increasing number of hydroxyl groups in the bile acid skeleton Homo sapiens ?
-
?
ursodeoxycholic acid + UDPglucuronate
-
Homo sapiens UDP + ursodeoxycholic acid O-glucuronide
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
chenodeoxycholic acid Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 8 substrate chenodeoxycholic acid Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.26
-
estradiol
-
Homo sapiens
0.28
-
bilirubin
-
Homo sapiens
2.2
-
testosterone
-
Homo sapiens