Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Sus scrofa | |
bilirubin | linear noncompetitive inhibition towards estrone | Sus scrofa | |
Cd2+ | complete inactivation at 10 mM | Sus scrofa | |
Cu2+ | 10 mM, complete inactivation | Sus scrofa | |
EDTA | not inhibited | Sus scrofa | |
estradiol | 17alpha or 17beta, noncompetitive inhibition towards estrone | Sus scrofa | |
estriol | linear noncompetitive inhibition towards estrone | Sus scrofa | |
Hg2+ | complete inactivation | Sus scrofa | |
additional information | dithioerythritol; not inhibitory: N-ethylmaleimide; product inhibition | Sus scrofa | |
p-chloromercuribenzoate | - |
Sus scrofa | |
Phenolphthalein | linear noncompetitive inhibition towards estrone | Sus scrofa | |
testosterone | linear noncompetitive inhibition towards estrone | Sus scrofa | |
UDP | noncompetitive inhibition towards UDPglucuronic acid at saturating concentration of UDPglucuronic acid, linear and uncompetitive inhibition with saturating concentration of estrone and varying concentration of UDPglucuronic acid | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
estrone | - |
Sus scrofa | |
0.025 | - |
UDPglucuronic acid | - |
Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Sus scrofa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, 30% activation | Sus scrofa | |
Mg2+ | 10 mM, 30% activation | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
pig | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
Storage Stability | Organism |
---|---|
-20°C, microsomal preparation, more than 4 months | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
estrone + UDPglucuronate | affinity increases during storage period at -20ºC for more than 4 months | Sus scrofa | UDP + estrone 3-O-glucuronide | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Sus scrofa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
2 | 45 | linear estrone glucuronide formation | Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
15 min, absence of substrates, slight inactivation | Sus scrofa |
48 | - |
15 min, 50% loss of activity | Sus scrofa |
70 | - |
15 min, complete inactivation | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | 8.7 | estrone | Sus scrofa |