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Literature summary for 2.4.1.149 extracted from

  • Basu, M.; Basu, S.
    Biosynthesis in vitro of Ii core glycosphingolipids from neolactotetraosylceramide by beta1-3- and beta1-6-N-acetylglucosaminyltransferases from mouse T-lymphoma (1984), J. Biol. Chem., 259, 12557-12562.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
detergent activates, reaction rate is optimal at detergent:protein ratio of 1.6 in Triton CF-54 or X-100, 1:3 in Nonidet P-40 Mus musculus
Nonidet P-40 reaction rate is optimal at detergent:protein ratio of 1:3 Mus musculus
Triton CF-54 reaction rate is optimal at detergent:protein ratio of 1:6 Mus musculus
Triton X-100 reaction rate is optimal at detergent:protein ratio of 1:6 Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 neolactotetraosylceramide
-
 Mus musculus
0.33
-
 UDP-N-acetyl-D-glucosamine solubilized enzyme Mus musculus
2.5
-
 UDP-N-acetyl-D-glucosamine membrane-bound enzyme Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Mus musculus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates, 48% as effective as Mn2+ Mus musculus
Co2+ activates, 52% as effective as Mn2+ Mus musculus
Mn2+ requirement, Km: 1.25 mM, Mn2+ cannot be replaced by Mg2+, Zn2+, Cu2+ or Cd2+ Mus musculus
additional information not activated by Mg2+, Zn2+, Cu2+ or Cd2+ Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus biosynthesis of Ii core glycosphingolipids ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
P-1798 cell lymphoma cell line Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information biosynthesis of Ii core glycosphingolipids Mus musculus ?
-
 ?
UDP-N-acetyl-D-glucosamine + asialo alpha1-acid glycoprotein sialic acid depleted alpha1-acid glycoprotein, 30% of the activity with neolactotetraosylceramide Mus musculus ?
-
 ?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. neolactotetraosylceramide, GlcNAcT-2, synthesis in vitro of Ii core glycosphingolipids Mus musculus UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 ?
UDP-N-acetyl-D-glucosamine + lactosylceramide 15% of the activity with neolactotetraosylceramide Mus musculus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Mus musculus