Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.149 extracted from

  • McAuliffe, J.C.; Ujita, M.; Fukuda, M.; Hindsgaul, O.
    Synthesis of selectively radiolabeled hexasaccharides for the determination of enzymatic regioselectivity (1999), Glycoconjugate J., 16, 767-772.
    View publication on PubMed
Search for more literature for 2.4.1.149

Cloned(Commentary)

Cloned (Comment) Organism
plasmid cDNA encoding the catalytic domain of i-GlcNAcT, amino acids 53-415, is expressed in COS-1 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + N-glycan Homo sapiens polylactosamine extension occurs on both beta1,2- and beta1,6-branches of complex N-type glycans ?
-
 ?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine Homo sapiens poly-N-acetyllactosamine biosynthesis, polylactosamine extension occurs on both beta1,2- and beta1,6-branches of complex N-type glycans ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-6)(Galbeta(1-4)GlcNAcbeta(1-2))Manalpha(1-6)Manbeta-octyl regioselectivity of enzyme, favored site of GlcNAc addition is the lower beta1,2-branch over the beta1,6-branch by a 3:1 ratio resulting in a mixture of heptasaccharides Homo sapiens ?
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta-p-nitrophenol
-
 Homo sapiens UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta-p-nitrophenol
-
 ?
UDP-N-acetyl-D-glucosamine + N-glycan polylactosamine extension occurs on both beta1,2- and beta1,6-branches of complex N-type glycans Homo sapiens ?
-
 ?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine poly-N-acetyllactosamine biosynthesis, polylactosamine extension occurs on both beta1,2- and beta1,6-branches of complex N-type glycans Homo sapiens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens