Cloned (Comment) | Organism |
---|---|
gene PAS_chr2-1_0212, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha | Komagataella pastoris |
gene PAS_chr2-1_0256, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha | Komagataella pastoris |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of Pichia pastoris single and all PMT knockout mutant strains, the DELTApmt1 and DELTApmt2 strains secrete protein with significantly reduced O-glycan site occupancy | Komagataella pastoris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-[[(3-(1-phenyl-2-hydroxy)ethoxy)-4-(2-phenylethoxy)phenyl]methylene]-4-oxo-2-thioxo-3-thiazolidine acetic acid | i.e. PMTi-3, inhibits PMT enzymes | Komagataella pastoris | |
5-[[(3-(1-phenyl-2-hydroxy)ethoxy)-4-(2-phenylethoxy)phenyl]methylene]-4-oxo-2-thioxo-3-thiazolidineacetic acid | i.e. PMTi-3, inhibits PMT enzymes | Komagataella pastoris | |
5-[[3,4-bis(phenylmethoxy)phenyl]methylene]-4-oxo-2-thioxo-3-thiazolidine acetic acid | i.e. PMTi-1, inhibits PMT enzymes | Komagataella pastoris | |
5-[[3,4-bis(phenylmethoxy)phenyl]methylene]-4-oxo-2-thioxo-3-thiazolidineacetic acid | i.e. PMTi-1, inhibits PMT enzymes | Komagataella pastoris | |
additional information | inhibitor synthesis, overview; inhibitor synthesis, overview | Komagataella pastoris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Komagataella pastoris | 5783 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataella pastoris | C4QZZ6 | formerly Pichia pastoris, several strains, gene PAS_chr2-1_0212, isozyme PMT1 | - |
Komagataella pastoris | C4R044 | formerly Pichia pastoris, several strains, gene PAS_chr2-1_0256, isozyme PMT2 | - |
Komagataella pastoris GS115 | C4QZZ6 | formerly Pichia pastoris, several strains, gene PAS_chr2-1_0212, isozyme PMT1 | - |
Komagataella pastoris GS115 | C4R044 | formerly Pichia pastoris, several strains, gene PAS_chr2-1_0256, isozyme PMT2 | - |
Synonyms | Comment | Organism |
---|---|---|
PMT1 | - |
Komagataella pastoris |
PMT2 | - |
Komagataella pastoris |
protein-Omannosyltransferase | - |
Komagataella pastoris |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the protein-O-mannosyltransferase (PMT) family. Pichia pastoris has five PMT genes. Based on sequence homology, the PMTs can be grouped into three subfamilies, with both PMT1 and PMT2 subfamilies possessing two members each (PMT1 and PMT5, and PMT2 and PMT6, respectively). The remaining subfamily, PMT4, has only one member, PMT4. PMT1 and PMT2 each play a significant role in O-glycosylation | Komagataella pastoris |
malfunction | PMT2 knockout, and to a lesser extent a PMT1 knockout, in addition to a reduction in O-glycan site occupancy also leads to shortened O-glycan chain lengths | Komagataella pastoris |
physiological function | protein O-mannosylation in fungi is initiated at the endoplasmic reticulum by a family of protein-O-mannosyltransferases that transfer mannose from dolichyl phosphate-activated mannose to serine or threonine residues of proteins entering the endoplasmic reticulum | Komagataella pastoris |