Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chymotrypsin | inactivation | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Rattus norvegicus | 16020 | - |
microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl phosphate D-mannose + ribonuclease 2 | Rattus norvegicus | C-mannosylation activity | dolichyl phosphate + ribonuclease 2-D-mannose | - |
? | |
dolichyl phosphate D-mannose + ribonuclease 2 | Rattus norvegicus | biosynthetic pathway | dolichyl phosphate + ribonuclease 2-D-mannose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
C-mannosylating enzyme | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl phosphate D-mannose + human ribonuclease 2 | whole protein or N-terminal dodecapeptide containing Trp7 | Rattus norvegicus | dolichyl phosphate + human ribonuclease 2-D-mannose | D-mannose is bound at Trp7 forming a C-C linkage | ? | |
dolichyl phosphate D-mannose + human ribonuclease 2 | C-mannosylation activity | Rattus norvegicus | dolichyl phosphate + human ribonuclease 2-D-mannose | D-mannose is bound at Trp7 forming a C-C linkage | ? | |
dolichyl phosphate D-mannose + human ribonuclease 2 | no C-mannosylation activity of Trp7 when Trp10 is exchanged for Ala in the acceptor substrate | Rattus norvegicus | dolichyl phosphate + human ribonuclease 2-D-mannose | D-mannose is bound at Trp7 forming a C-C linkage | ? | |
dolichyl phosphate D-mannose + human ribonuclease 2 | recombinant wild-type RNase from E. coli is no substrate | Rattus norvegicus | dolichyl phosphate + human ribonuclease 2-D-mannose | D-mannose is bound at Trp7 forming a C-C linkage | ? | |
dolichyl phosphate D-mannose + ribonuclease 2 | C-mannosylation activity | Rattus norvegicus | dolichyl phosphate + ribonuclease 2-D-mannose | - |
? | |
dolichyl phosphate D-mannose + ribonuclease 2 | C-mannosylation activity | Rattus norvegicus | dolichyl phosphate + ribonuclease 2-D-mannose | D-mannose is bound at Trp7 forming a C-C linkage | ? | |
dolichyl phosphate D-mannose + ribonuclease 2 | biosynthetic pathway | Rattus norvegicus | dolichyl phosphate + ribonuclease 2-D-mannose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | - |
about | Rattus norvegicus |
37 | - |
assay at | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | - |
22 h, stable | Rattus norvegicus |
37 | - |
60 min, stable | Rattus norvegicus |
95 | - |
inactivation | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Rattus norvegicus |