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Literature summary for 2.4.1.109 extracted from

  • Babczinski, P.; Haselbeck, A.; Tanner, W.
    Yeast mannosyl transferases requiring dolichyl phosphate and dolichyl phosphate mannose as substrate. Partial purification and characterization of the solubilized enzyme (1980), Eur. J. Biochem., 105, 509-515.
    View publication on PubMed
Search for more literature for 2.4.1.109

Activating Compound

Activating Compound Comment Organism Structure
deoxycholate 0.025%, stimulates slightly Saccharomyces cerevisiae
additional information not affected by Triton X-100 up to 0.2% Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
additional information not affected by Triton X-100 up to 0.2% Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound Saccharomyces cerevisiae 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+ or Mn2+ stimulates Saccharomyces cerevisiae
Mg2+ optimal stimulation at 7 mM Saccharomyces cerevisiae
Mn2+ Mg2+ or Mn2+ stimulates Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dolichyl phosphate D-mannose + protein Saccharomyces cerevisiae production of cell-wall mannoproteins dolichyl phosphate + O-D-mannosylprotein
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00057
-
 partially purified enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dolichyl phosphate D-mannose + protein
-
 Saccharomyces cerevisiae dolichyl phosphate + O-D-mannosylprotein
-
 ?
dolichyl phosphate D-mannose + protein the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues Saccharomyces cerevisiae dolichyl phosphate + O-D-mannosylprotein
-
 ir
dolichyl phosphate D-mannose + protein acceptor substrate: HCl-treated cell wall mannoprotein from Saccharomyces cerevisiae Saccharomyces cerevisiae dolichyl phosphate + O-D-mannosylprotein
-
 ir
dolichyl phosphate D-mannose + protein production of cell-wall mannoproteins Saccharomyces cerevisiae dolichyl phosphate + O-D-mannosylprotein
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.7
-
-
 Saccharomyces cerevisiae