Cloned (Comment) | Organism |
---|---|
gene fft, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Pichia pastoris | Viguiera discolor |
gene fft-1, sequence comparisons, recombinant expression in Pichia pastoris | Helianthus tuberosus |
Protein Variants | Comment | Organism |
---|---|---|
H308T | site-directed mutagenesis | Viguiera discolor |
M19F | site-directed mutagenesis | Viguiera discolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Helianthus tuberosus | inulins, one of the fructan types with beta(2-1)-linkages, are elongated by fructan:fructan1-fructosyltransferase (1-FFT) using a fructosyl unit from a donor inulin to elongate the acceptor inulin molecule | ? | - |
? | |
additional information | Viguiera discolor | inulins, one of the fructan types with beta(2-1)-linkages, are elongated by fructan:fructan1-fructosyltransferase (1-FFT) using a fructosyl unit from a donor inulin to elongate the acceptori inulin molecule | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helianthus tuberosus | O81985 | gene fft-1 | - |
Viguiera discolor | Q2WEC6 | gene fft | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | inulins, one of the fructan types with beta(2-1)-linkages, are elongated by fructan:fructan1-fructosyltransferase (1-FFT) using a fructosyl unit from a donor inulin to elongate the acceptor inulin molecule | Helianthus tuberosus | ? | - |
? | |
additional information | inulins, one of the fructan types with beta(2-1)-linkages, are elongated by fructan:fructan1-fructosyltransferase (1-FFT) using a fructosyl unit from a donor inulin to elongate the acceptori inulin molecule | Viguiera discolor | ? | - |
? | |
additional information | 1-kestotriose is used as sole substrate, product analysis of wild-type and mutant enzyme reactions, overview | Viguiera discolor | ? | - |
? | |
additional information | 1-kestotriose is used as sole substrate, product analysis, overview | Helianthus tuberosus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1-FFT | - |
Viguiera discolor |
1-FFT | - |
Helianthus tuberosus |
fructan:fructan 1-fructosyltransferase | - |
Viguiera discolor |
fructan:fructan 1-fructosyltransferase | - |
Helianthus tuberosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
recombinant enzyme, 5 min, inactivation | Viguiera discolor |
90 | - |
recombinant enzyme, 5 min, inactivation | Helianthus tuberosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Viguiera discolor |
5 | - |
assay at | Helianthus tuberosus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the glycoside hydrolase family 32 | Viguiera discolor |
evolution | the enzyme is a member of the glycoside hydrolase family 32 | Helianthus tuberosus |
metabolism | inulin synthesis is the result of the combined action of sucrose:sucrose 1-fructosyltransferase, 1-SST, EC 2.4.1.99, and fructan:fructan 1-fructosyltransferase, 1-FFT, where 1-SST catalyzes the formation of 1-kestotriose from two sucrose molecules, while releasing glucose. Secondly 1-FFT transfers fructosyl units from one inulin molecule to another to elongate the inulin chain | Helianthus tuberosus |
additional information | three-dimensional structure and homology structure modeling based on the resolved three-dimensional structure of 6-SST/6-SFT from Pachysandra terminalis, overview | Helianthus tuberosus |
additional information | three-dimensional structure and homology structure modeling, based on the resolved three-dimensional structure of 6-SST/6-SFT from Pachysandra terminalis, overview | Viguiera discolor |