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Literature summary for 2.3.3.9 extracted from
- Operation of glyoxylate cycle in halophilic archaea: presence of malate synthase and isocitrate lyase in Haloferax volcanii (1998), FEBS Lett., 434, 13-16.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00011 | - |
glyoxylate | pH 8.0, 40°C | Haloferax volcanii |  |
| 0.00012 | - |
acetyl-CoA | pH 8.0, 40°C | Haloferax volcanii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | salt optimum: 3.0 M KCl | Haloferax volcanii | |
| Mg2+ | activity is dependent on Mg2+ | Haloferax volcanii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
3 * 67000, SDS-PAGE | Haloferax volcanii |
| 200000 | - |
gel filtration | Haloferax volcanii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + H2O + glyoxylate | Haloferax volcanii | key enzyme of the glyoxylate cycle enzyme | (S)-malate + CoA | - |
? | |
| acetyl-CoA + H2O + glyoxylate | Haloferax volcanii DSM 3757 | key enzyme of the glyoxylate cycle enzyme | (S)-malate + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haloferax volcanii | - |
- |
- |
| Haloferax volcanii DSM 3757 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Haloferax volcanii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:acetate-grown cell | no activity can be detected when lactate is the carbon source, but high activity is measured when acetate is | Haloferax volcanii | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + H2O + glyoxylate | - |
Haloferax volcanii | (S)-malate + CoA | - |
? | |
| acetyl-CoA + H2O + glyoxylate | key enzyme of the glyoxylate cycle enzyme | Haloferax volcanii | (S)-malate + CoA | - |
? | |
| acetyl-CoA + H2O + glyoxylate | - |
Haloferax volcanii DSM 3757 | (S)-malate + CoA | - |
? | |
| acetyl-CoA + H2O + glyoxylate | key enzyme of the glyoxylate cycle enzyme | Haloferax volcanii DSM 3757 | (S)-malate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotrimer | 3 * 67000, SDS-PAGE | Haloferax volcanii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at | Haloferax volcanii |
| 50 | 60 | - |
Haloferax volcanii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 80 | 25°C: about 45% of maximal activity, 80°C: about 45% of maximal activity | Haloferax volcanii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Haloferax volcanii |
| 8 | 9 | - |
Haloferax volcanii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | pH 7.0: about 40% of maximal activity, pH 10.0: about 45% of maximal activity | Haloferax volcanii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | key enzyme of the glyoxylate cycle enzyme | Haloferax volcanii |
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