Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.3.9 extracted from

  • Bracken, C.D.; Neighbor, A.M.; Lamlenn, K.K.; Thomas, G.C.; Schubert, H.L.; Whitby, F.G.; Howard, B.R.
    Crystal structures of a halophilic archaeal malate synthase from Haloferax volcanii and comparisons with isoforms A and G (2011), BMC Struct. Biol., 11, 23.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.3.9

Crystallization (Commentary)

Crystallization (Comment) Organism
malate synthase isoform H in complex with glyoxylate and also as a ternary complex with acetyl-coenzyme A and pyruvate, sitting drop vapor diffusion method, using 0.17 M ammonium acetate, 24.5-27% (w/v) PEG 4500, 15% (v/v) glycerol, and 0.085 M sodium acetate trihydrate at a pH of 4.4-5.0 Haloferax volcanii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ contains one Mg2+ ion in the active site Haloferax volcanii

Organism

Organism UniProt Comment Textmining
Haloferax volcanii D4GTL2
-
-

Purification (Commentary)

Purification (Comment) Organism
reverse phase chromatography, anion-exchange column chromatography, and gel filtration Haloferax volcanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glyoxylate + acetyl-CoA + H2O
-
 Haloferax volcanii (S)-malate + CoA
-
 ?

Subunits

Subunits Comment Organism
monomer malate synthase isoforms A and G Haloferax volcanii
trimer or hexamer malate synthase isoform H is found in the native state as a trimer/hexamer equilibrium Haloferax volcanii

Synonyms

Synonyms Comment Organism
malate synthase A isoform Haloferax volcanii
malate synthase G isoform Haloferax volcanii
malate synthase H isoform Haloferax volcanii
MSA
-
 Haloferax volcanii
MSG
-
 Haloferax volcanii
MSH
-
 Haloferax volcanii