Cloned (Comment) | Organism |
---|---|
cloning of His-tagged fusion protein of citrate synthase and mitochondrial malate dehydrogenase, expression in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | no channeling of oxaloacetate between malate dehydrogenase and citrate synthase in a recombinant fusion protein using a coupled assay | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
recombinant fusion protein of yeast citrate synthase and mitochondrial malate dehydrogenase | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged fusion protein of citrate synthase and mitochondrial malate dehydrogenase from E. coli | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + H2O + oxaloacetate = citrate + CoA | free diffusion mechanism, no channeling of oxaloacetate from malate dehydrogenase | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
213 | - |
purified fusion protein of citrate synthase and mitochondrial malate dehydrogenase, coupled assay | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | no channeling of oxaloacetate between malate dehydrogenase and citrate synthase in a recombinant fusion protein using a coupled assay | Saccharomyces cerevisiae | citrate + CoA | - |
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