General Stability | Organism |
---|---|
very instable without glycerol | Priestia megaterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 10 mM, 64% inhibition; 64% inhibition at 10 mM | Priestia megaterium | |
AMP | 10 mM, 36% inhibition; 36% inhibition at 10 mM | Priestia megaterium | |
ATP | 10 mM, 79% inhibition; 79% inhibition at 10 mM | Priestia megaterium | |
NAD+ | 10 mM, 9% inhibition | Priestia megaterium | |
NADH | 10 mM, 31% inhibition; 1-10 mM; non-specific, 31% inhibition at 10 mM | Priestia megaterium | |
NADP+ | 10 mM, 32% inhibition | Priestia megaterium | |
NADPH | 10 mM, 57% inhibition; 57% inhibition at 10 mM | Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
oxaloacetate | - |
Priestia megaterium | |
0.076 | - |
acetyl-CoA | - |
Priestia megaterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40300 | - |
2 * 40300, SDS-PAGE, gel filtration in guanidine-HCl, amino acid composition | Priestia megaterium |
40300 | - |
2 * 40300, SDS-PAGE, gel filtration in guanidinium chloride | Priestia megaterium |
84000 | - |
gel filtration | Priestia megaterium |
84000 | - |
Stokes radius, sedimentation coefficient | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Priestia megaterium D101 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Priestia megaterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.6 | - |
- |
Priestia megaterium |
21.6 | - |
purified enzyme | Priestia megaterium |
Storage Stability | Organism |
---|---|
4°C, pure citrate synthase is completely stable for at least 6 months | Priestia megaterium |
4°C, Tris-HCl buffer, pH 8.0, 20% v/v glycerol and 0.1 M KCl, at least 6 months | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | - |
Priestia megaterium | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | - |
Priestia megaterium D101 | citrate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40300, SDS-PAGE, gel filtration in guanidine-HCl, amino acid composition | Priestia megaterium |
dimer | 2 * 40300, SDS-PAGE, gel filtration in guanidinium chloride | Priestia megaterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
enzyme assay at | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
enzyme assay at | Priestia megaterium |