Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C93S | sumoylatzion-defective mutant | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
SUMO-1-conjugating enzyme Ubc9 | - |
Homo sapiens |
Ubc9 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | reduction of Ubc9 protein levels by small interfering RNA attenuates hormonal activation of a mineralocorticoid receptor reporter construct as well as an endogenous target gene by mineralocorticoid receptor. A sumoylation-inactive mutant Ubc9 (C93S) similarly interacts with mineralocorticoid receptor and potentiates aldosterone-dependent mineralocorticoid receptor transactivation. An mineralocorticoid receptor mutant in which four lysine residues within sumoylation motifs are mutated into arginine (K89R/K399R/K494R/K953R) fails to be sumoylated, but Ubc9 similarly enhances transactivation by the mutant mineralocorticoid receptor. Coexpression of Ubc9 and steroid receptor coactivator SRC-1 synergistically enhances mineralocorticoid receptor-mediated transactivation in transient transfection assays | Homo sapiens |