Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.2.23 extracted from

  • Nguyen, L.; Plafker, K.S.; Starnes, A.; Cook, M.; Klevit, R.E.; Plafker, S.M.
    The ubiquitin-conjugating enzyme, UbcM2, is restricted to monoubiquitylation by a two-fold mechanism that involves backside residues of E2 and Lys48 of ubiquitin (2014), Biochemistry, 53, 4004-4014.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.23

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
K86V during transfer of ubiquitin to the final substrate or E3 ligase, reaction of EC 2.3.2.27, mutant shows increased polyubiquitin chain building activity with ubiquitin mutant K48R Homo sapiens
N80Q during transfer of ubiquitin to the final substrate or E3 ligase, reaction of EC 2.3.2.27, mutant shows increased polyubiquitin chain building activity with ubiquitin mutant K48R Homo sapiens
P79A during transfer of ubiquitin to the final substrate or E3 ligase, reaction of EC 2.3.2.27, mutant shows a much reduced capacity to ubiquitylate RING-E3 enzyme RNF25 Homo sapiens
R94Q/L98M during transfer of ubiquitin to the final substrate or E3 ligase, reaction of EC 2.3.2.27, mutant shows increased polyubiquitin chain building activity with ubiquitin mutant K48R Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q969T4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information during transfer of ubiquitin to the final substrate or E3 ligase, reaction of EC 2.3.2.27, enzyme is restricted to monoubiquitinylation. UbcM2 shows enhanced polyubiquitin synthesizing activity in reaction mixtures containing ubiquitin mutant K48R. In contrast, reaction mixtures containing ubiquitin mutant K6R show a mild suppression of UbcM2 activity Homo sapiens ?
-
 ?