Application | Comment | Organism |
---|---|---|
drug development | UbcA1 shows antitumor properties, a potential anticancer drug candidate | Cyclocybe aegerita |
Cloned (Comment) | Organism |
---|---|
subcloned in Escherichia coli BL21(DE2), via pET28 vector, N-terminally His-tagged | Cyclocybe aegerita |
Crystallization (Comment) | Organism |
---|---|
recombinant enzyme, N-terminally His-tagged, subcloned in Escherichia coli BL21(DE2), via pET28 vector, purified using Ni-NTA column and HiLoad 16/60 Superdex 75 gel filtration column, diffracted X-rays to 1.7 A. The crystal belongs to space group C2 | Cyclocybe aegerita |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
2 * 18000, single band on SDS-PAGE, indicating a dimeric form. Gel filtration results in an estimated molecular weight of 42 kDa | Cyclocybe aegerita |
42000 | - |
gel filtration results in an estimated molecular weight of 42 kDa | Cyclocybe aegerita |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyclocybe aegerita | - |
medicinal mushroom, basidiomycetes | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | ubiquitination is involved in myriad cell and disease pathways. The ubiquitin-conjugating E2 is the central player in the ubiquitin-transfer pathway | Cyclocybe aegerita |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, N-terminally His-tagged, subcloned in Escherichia coli BL21(DE2), via pET28 vector, purified using Ni-NTA column and HiLoad 16/60 Superdex 75 gel filtration column, purity 95% after two steps | Cyclocybe aegerita |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 18000, single band on SDS-PAGE, indicating a dimeric form. Gel filtration results in an estimated molecular weight of 42 kDa | Cyclocybe aegerita |
Synonyms | Comment | Organism |
---|---|---|
UbcA1 | - |
Cyclocybe aegerita |
ubiquitin-conjugating enzyme E2 | - |
Cyclocybe aegerita |
General Information | Comment | Organism |
---|---|---|
physiological function | ubiquitination is involved in myriad cell and disease pathways. The ubiquitin-conjugating E2 is the central player in the ubiquitin-transfer pathway | Cyclocybe aegerita |