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Literature summary for 2.3.2.23 extracted from

  • Tokgoez, Z.; Bohnsack, R.N.; Haas, A.L.
    Pleiotropic effects of ATP*Mg2+ binding in the catalytic cycle of ubiquitin-activating enzyme (2006), J. Biol. Chem., 281, 14729-14737.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000073
-
[ubiquitin-carrier-protein Ubc2b]-L-cysteine mutant D576N, pH 7.5, temperature not specified in the publication Homo sapiens
0.000089
-
[ubiquitin-carrier-protein Ubc2b]-L-cysteine mutant K528A, pH 7.5, temperature not specified in the publication Homo sapiens
0.000111
-
[ubiquitin-carrier-protein Ubc2b]-L-cysteine wild-type, pH 7.5, temperature not specified in the publication Homo sapiens
0.000122
-
[ubiquitin-carrier-protein Ubc2b]-L-cysteine mutant D576E, pH 7.5, temperature not specified in the publication Homo sapiens
0.000135
-
[ubiquitin-carrier-protein Ubc2b]-L-cysteine mutant D576A, pH 7.5, temperature not specified in the publication Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information kinetics for Uba1a-catalyzed transthiolation of Ubc2b are used as a reporter assay for determining the Km and kcat values for the three cosubstrates of the ubiquitin-activating enzyme. The E2 transthiolation assays are more sensitive to the potential presence of trace catalytically active fragments than the single turnover end point assays used for quantitating ternary complex stoichiometry Homo sapiens ?
-
?
[ubiquitin-activating protein Uba1a]-S-ubiquitinyl-L-cysteine + [ubiquitin-carrier-protein Ubc2b]-L-cysteine
-
Homo sapiens [ubiquitin-activating protein Uba1a]-L-cysteine + [ubiquitin-carrier-protein Ubc2b]-S-ubiquitinyl-L-cysteine
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01 1 [ubiquitin-carrier-protein Ubc2b]-L-cysteine mutant K528A, pH 7.5, temperature not specified in the publication Homo sapiens
0.022
-
[ubiquitin-carrier-protein Ubc2b]-L-cysteine mutant D576N, pH 7.5, temperature not specified in the publication Homo sapiens
0.1
-
[ubiquitin-carrier-protein Ubc2b]-L-cysteine mutant D576E, pH 7.5, temperature not specified in the publication Homo sapiens
0.12
-
[ubiquitin-carrier-protein Ubc2b]-L-cysteine mutant D576A, pH 7.5, temperature not specified in the publication Homo sapiens
3.4
-
[ubiquitin-carrier-protein Ubc2b]-L-cysteine wild-type, pH 7.5, temperature not specified in the publication Homo sapiens