Cloned (Comment) | Organism |
---|---|
overexpression as His-tagged protein in Escherichia coli JM109, DNA sequence analysis | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of transgenic Nicotiana tabacum plants via Agrobacterium tumefaciens transformation, functional extracellular overexpression of the Arabidopsis thaliana enzyme in leaves, transgenic and control plants show the same amount of glutathione degradation | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-nitroaniline | strong, competitive against glutathione | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
glutathione | - |
Arabidopsis thaliana | |
0.8 | - |
L-Glu-4-nitroanilide | recombinant enzyme | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | recombinant enzyme is extracellular in transgenic tabacco plants | Arabidopsis thaliana | - |
- |
membrane | membrane-bound | Arabidopsis thaliana | 16020 | - |
vacuole | membrane-bound | Arabidopsis thaliana | 5773 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid sequence alignment, enzyme gene family | Arabidopsis thaliana |
additional information | - |
amino acid sequence alignment, enzyme gene family | Homo sapiens |
additional information | - |
amino acid sequence alignment, enzyme gene family | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + an amino acid | Arabidopsis thaliana | initial step of glutathione degradation | L-cysteinylglycine + a 5-L-glutamyl-amino acid | - |
? | |
L-Glu-4-nitroanilide + glutathione | Arabidopsis thaliana | L-Glu-4-nitroanilide is a suicide substrate | 4-nitroaniline + 5-L-glutamyl-glutathione | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana | O65652 | - |
- |
Arabidopsis thaliana | Q39078 | - |
- |
Arabidopsis thaliana | Q9M0G0 | - |
- |
Homo sapiens | P19440 | - |
- |
Mus musculus | Q60928 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from E. coli | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5-L-glutamyl)-peptide + acceptor + H+ | - |
Arabidopsis thaliana | peptide + 5-L-glutamyl amino acid | - |
? | |
5-D-glutamyl-4-nitroanilide + acceptor | - |
Arabidopsis thaliana | 4-nitroaniline + 5-D-glutamyl-acceptor | - |
? | |
5-L-glutamyl-4-nitroanilide + glycylglycine | - |
Arabidopsis thaliana | 4-nitroaniline + 5-L-glutamylglycylglycine | - |
? | |
5-L-glutamyl-7-amido-4-methylcoumarin + acceptor | - |
Arabidopsis thaliana | 7-amino-4-methylcoumarin + 5-L-glutamyl-acceptor | - |
? | |
glutathione + an amino acid | initial step of glutathione degradation | Arabidopsis thaliana | L-cysteinylglycine + a 5-L-glutamyl-amino acid | - |
? | |
L-Glu-4-nitroanilide + glutathione | L-Glu-4-nitroanilide is a suicide substrate | Arabidopsis thaliana | 4-nitroaniline + 5-L-glutamyl-glutathione | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
4-nitroaniline | - |
Arabidopsis thaliana |