Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Maleate | - |
Rattus norvegicus | |
Maleate | stimulates hydrolase reaction 3fold, inhibits transpeptidase reaction, with L-Glu as substrate | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-diazo-5-oxo-L-norleucine | 5-glutamyl-donor, protect transpeptidase and hydrolase activity, not 5-carbon derivative; i.e. DON, strong, irreversible modification of glutamyl-binding site of light subunit | Homo sapiens | |
6-diazo-5-oxo-L-norleucine | - |
Rattus norvegicus | |
L-azaserine | irreversible inactivation; not 5-carbon derivative; transpeptidase and hydrolase reaction, glutamyl-donor protects | Homo sapiens | |
L-azaserine | irreversible inactivation | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid composition | Homo sapiens |
additional information | - |
amino acid composition | Rattus norvegicus |
22000 | - |
1 * 62000 + 1 * 22000, SDS-PAGE | Homo sapiens |
62000 | - |
1 * 62000 + 1 * 22000, SDS-PAGE | Homo sapiens |
86000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
glycoprotein | - |
Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Homo sapiens | - |
kidney | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
800 | - |
purified enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5-L-glutamyl)-peptide + acceptor + H+ | Glu-donor is 5-L-Glu-4-nitroanilide | Homo sapiens | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | Glu-donor is 5-L-Glu-4-nitroanilide | Rattus norvegicus | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | acceptors are amino acids or dipeptides | Homo sapiens | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | acceptors are amino acids or dipeptides | Rattus norvegicus | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | acceptor specificity, overview | Homo sapiens | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | acceptor specificity, overview | Rattus norvegicus | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | Glu-donor is GSH | Homo sapiens | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | Glu-donor is GSH | Rattus norvegicus | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donor: S-acetophenoneglutathione | Homo sapiens | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donor: S-acetophenoneglutathione | Rattus norvegicus | peptide + 5-L-glutamyl amino acid | - |
? | |
(5-L-glutamyl)-peptide + acceptor + H+ | donor: S-acetylglutathione | Homo sapiens | peptide + 5-L-glutamyl amino acid | - |
? | |
5-L-glutamyl-4-nitroanilide + glycylglycine | - |
Homo sapiens | 4-nitroaniline + 5-L-glutamylglycylglycine | - |
? | |
5-L-glutamyl-4-nitroanilide + glycylglycine | - |
Rattus norvegicus | 4-nitroaniline + 5-L-glutamylglycylglycine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-alanine | - |
Homo sapiens | 4-nitroaniline + 5-L-glutamyl-L-alanine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-alanine | - |
Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-alanine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-asparagine | - |
Homo sapiens | 4-nitroaniline + 5-L-glutamyl-L-asparagine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-glutamate | - |
Homo sapiens | 4-nitroaniline + 5-L-glutamyl-L-glutamate | - |
? | |
5-L-glutamyl-4-nitroanilide + L-glutamate | - |
Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-glutamate | - |
? | |
5-L-glutamyl-4-nitroanilide + L-glutamine | - |
Homo sapiens | 4-nitroaniline + 5-L-glutamyl-L-glutamine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-glutamine | - |
Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-glutamine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-methionine | - |
Homo sapiens | 4-nitroaniline + 5-L-glutamyl-L-methionine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-methionine | - |
Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-methionine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-serine | - |
Homo sapiens | 4-nitroaniline + 5-L-glutamyl-L-serine | - |
? | |
5-L-glutamyl-4-nitroanilide + L-serine | - |
Rattus norvegicus | 4-nitroaniline + 5-L-glutamyl-L-serine | - |
? | |
glutathione + acceptor | - |
Homo sapiens | cysteinylglycine + 5-L-glutamyl-acceptor | - |
? | |
glutathione + acceptor | - |
Rattus norvegicus | cysteinylglycine + 5-L-glutamyl-acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Rattus norvegicus |
dimer | 1 * 62000 + 1 * 22000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1150 | - |
4-nitroanilide | - |
Homo sapiens | |
1250 | - |
4-nitroanilide | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
additional information | - |
several isozymic forms with different content of sialic acid | Homo sapiens |