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Literature summary for 2.3.2.2 extracted from

  • Takahashi, S.; Zukin, R.S.; Steinman, H.M.
    gamma-Glutamyl transpeptidase from WI-38 fibroblasts: purification and active site modification studies (1981), Arch. Biochem. Biophys., 207, 87-95.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5-Iodoacetamidofluorescein inactivation, active site modification Homo sapiens
6-diazo-5-oxo-L-norleucine complete inactivation, accelerated by maleate, prevented by S-methylglutathione; i.e. DON, strong, irreversible modification of glutamyl-binding site of light subunit; maleate accelerates inactivation; S-methylglutathione Homo sapiens
L-azaserine
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
L-Glu-4-nitroanilide
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Homo sapiens 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
amino acid composition Homo sapiens
additional information
-
in the presence of Triton X-100: molecular aggregation Homo sapiens
additional information
-
protease solubilized enzymes have a reduced MW Homo sapiens
20000
-
1 * 53000 + 1 * 20000, papain-solubilized, SDS-PAGE Homo sapiens
20000
-
1 * 62000 + 1 * 20000, SDS-PAGE Homo sapiens
53000
-
1 * 53000 + 1 * 20000, papain-solubilized, SDS-PAGE Homo sapiens
62000
-
1 * 62000 + 1 * 20000, SDS-PAGE Homo sapiens
71000
-
papain-solubilized, gel filtration Homo sapiens
82000
-
papain-solubilized enzyme, gel filtration Homo sapiens
160000
-
Triton X-100 solubilized, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Homo sapiens
-
lung
-
Homo sapiens
-
lung fibroblast WI-38 fetal Homo sapiens
-
WI-38 cell whole body, fetal Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16
-
purified enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-L-glutamyl)-peptide + acceptor + H+
-
Homo sapiens peptide + 5-L-glutamyl amino acid
-
?
5-L-glutamyl-4-nitroanilide + glycylglycine
-
Homo sapiens 4-nitroaniline + 5-L-glutamylglycylglycine
-
?
L-Glu-4-nitroanilide + glutathione
-
Homo sapiens 4-nitroaniline + 5-L-glutamyl-glutathione
-
?

Subunits

Subunits Comment Organism
dimer 1 * 53000 + 1 * 20000, papain-solubilized, SDS-PAGE Homo sapiens
dimer 1 * 62000 + 1 * 20000, SDS-PAGE Homo sapiens
More protease solubilization leads to a reduced MW of the larger subunit Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Homo sapiens