Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-Iodoacetamidofluorescein | inactivation, active site modification | Homo sapiens | |
6-diazo-5-oxo-L-norleucine | complete inactivation, accelerated by maleate, prevented by S-methylglutathione; i.e. DON, strong, irreversible modification of glutamyl-binding site of light subunit; maleate accelerates inactivation; S-methylglutathione | Homo sapiens | |
L-azaserine | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
L-Glu-4-nitroanilide | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid composition | Homo sapiens |
additional information | - |
in the presence of Triton X-100: molecular aggregation | Homo sapiens |
additional information | - |
protease solubilized enzymes have a reduced MW | Homo sapiens |
20000 | - |
1 * 53000 + 1 * 20000, papain-solubilized, SDS-PAGE | Homo sapiens |
20000 | - |
1 * 62000 + 1 * 20000, SDS-PAGE | Homo sapiens |
53000 | - |
1 * 53000 + 1 * 20000, papain-solubilized, SDS-PAGE | Homo sapiens |
62000 | - |
1 * 62000 + 1 * 20000, SDS-PAGE | Homo sapiens |
71000 | - |
papain-solubilized, gel filtration | Homo sapiens |
82000 | - |
papain-solubilized enzyme, gel filtration | Homo sapiens |
160000 | - |
Triton X-100 solubilized, gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Homo sapiens | - |
lung | - |
Homo sapiens | - |
lung fibroblast | WI-38 fetal | Homo sapiens | - |
WI-38 cell | whole body, fetal | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16 | - |
purified enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5-L-glutamyl)-peptide + acceptor + H+ | - |
Homo sapiens | peptide + 5-L-glutamyl amino acid | - |
? | |
5-L-glutamyl-4-nitroanilide + glycylglycine | - |
Homo sapiens | 4-nitroaniline + 5-L-glutamylglycylglycine | - |
? | |
L-Glu-4-nitroanilide + glutathione | - |
Homo sapiens | 4-nitroaniline + 5-L-glutamyl-glutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 53000 + 1 * 20000, papain-solubilized, SDS-PAGE | Homo sapiens |
dimer | 1 * 62000 + 1 * 20000, SDS-PAGE | Homo sapiens |
More | protease solubilization leads to a reduced MW of the larger subunit | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Homo sapiens |