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Literature summary for 2.3.2.13 extracted from

  • Fang, J.Y.; Tan, S.J.; Yang, Z.; Tayag, C.; Han, B.
    Tumor bioengineering using a transglutaminase crosslinked hydrogel (2014), PLoS ONE, 9, e105616.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.13

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 20 mM MES at pH 6.8, 200 mM sodium chloride, 20 mM MgCl2, 6% (w/v) PEG 3350, 5 mM dithiothreitol, and 24% (v/v) glycerol Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
GTP
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78520
-
 1 * 78520, including the C-terminal His-tag and GTP, calculated from amino acid sequence Homo sapiens
78570
-
 1 * 78570, multiangle light scattering Homo sapiens
80000
-
 1 * 80000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[protein]-L-glutamine + alkylamine Homo sapiens
-
 [protein]-N5-alkyl-L-glutamine + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity resin column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[protein]-L-glutamine + alkylamine
-
 Homo sapiens [protein]-N5-alkyl-L-glutamine + NH3
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 80000, SDS-PAGE Homo sapiens
monomer 1 * 78520, including the C-terminal His-tag and GTP, calculated from amino acid sequence Homo sapiens
monomer 1 * 78570, multiangle light scattering Homo sapiens

Synonyms

Synonyms Comment Organism
TG2
-
 Homo sapiens
transglutaminase2
-
 Homo sapiens