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Literature summary for 2.3.2.13 extracted from
- biochemical characterization of medaka (Oryzias latipes) transglutaminases, OlTGK1 and OlTGK2, as orthologues of human keratinocyte-type transglutaminase (2015), PLoS ONE, 10, e0144194.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3)LysS cells | Oryzias latipes |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Oryzias latipes |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
x * 80000, isoform TGK1, SDS-PAGE | Oryzias latipes |
| 100000 | - |
x * 100000, isoform TGK2, SDS-PAGE | Oryzias latipes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [protein]-L-glutamine + alkylamine | Oryzias latipes | - |
[protein]-N5-alkyl-L-glutamine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryzias latipes | A0A0S3Q197 | - |
- |
| Oryzias latipes | A0A0S3Q1B1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Talon column chromatography and Superdex 200 gel filtration | Oryzias latipes |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epidermis | - |
Oryzias latipes | - |
| intervertebral disk | - |
Oryzias latipes | - |
| pancreas | isoform TGK1 | Oryzias latipes | - |
| pharyngeal tooth | - |
Oryzias latipes | - |
| skin | - |
Oryzias latipes | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [casein]-L-glutamine + biotin-labeled pentylamine | - |
Oryzias latipes | ? + NH3 | - |
? | |
| [protein]-L-glutamine + alkylamine | - |
Oryzias latipes | [protein]-N5-alkyl-L-glutamine + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 100000, isoform TGK2, SDS-PAGE | Oryzias latipes |
| ? | x * 80000, isoform TGK1, SDS-PAGE | Oryzias latipes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TGK1 | isoform | Oryzias latipes |
| TGK2 | isoform | Oryzias latipes |
| transglutaminase | - |
Oryzias latipes |
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Release 2023.1 (January 2023)
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