Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.2.13 extracted from

  • Yokoyama, K.; Utsumi, H.; Nakamura, T.; Ogaya, D.; Shimba, N.; Suzuki, E.; Taguchi, S.
    Screening for improved activity of a transglutaminase from Streptomyces mobaraensis created by a novel rational mutagenesis and random mutagenesis (2010), Appl. Microbiol. Biotechnol., 87, 2087-2096.
    View publication on PubMed
Search for more literature for 2.3.2.13

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is secreted as a pro-enzyme which is activated by the endoprotease TAMEP Streptomyces mobaraensis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Corynebacterum glutamicum strain ATTC13869 Streptomyces mobaraensis

Protein Variants

Protein Variants Comment Organism
A10S the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
D14N the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
D3F the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
D3L the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
D3N the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
E28D the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
E58D the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
H289F the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
H289Y the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
M16T the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
M16T/G283S the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
N320D the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
N32D the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
N32D/E264D/N320T the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
P12S the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
Q74A the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
Q74L the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
Q74N the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
R238F the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
R238L the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
R26F the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
R26L the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
R5K the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
S199A the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
S284T the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
S299L the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
S303A the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
S303F the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
S303T the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
T77A the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
T77F the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
T77L the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
T77S the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
V30D the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
V30I the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
V30T the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
V65I the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
V6T the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
W59F the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
Y34F the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
Y34F/D268N the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
Y42H the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
Y75A the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
Y75F the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis
Y75H the mutant shows higher specific activity compared to the wild type enzyme Streptomyces mobaraensis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on Streptomyces mobaraensis

Organism

Organism UniProt Comment Textmining
Streptomyces mobaraensis
-
-
-
Streptomyces mobaraensis s-8112
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Resource S column chromatography and Sephadex G25 gel filtration Streptomyces mobaraensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27.2
-
 wild type enzyme, at pH 6.0 and 37°C Streptomyces mobaraensis
27.4
-
 mutant enzyme N320D, at pH 6.0 and 37°C Streptomyces mobaraensis
28.5
-
 mutant enzyme Y42H, at pH 6.0 and 37°C Streptomyces mobaraensis
28.6
-
 mutant enzyme Y34F/D268N, at pH 6.0 and 37°C Streptomyces mobaraensis
28.7
-
 mutant enzyme N32D/E264D/N320T, at pH 6.0 and 37°C Streptomyces mobaraensis
28.9
-
 mutant enzyme N32D, at pH 6.0 and 37°C Streptomyces mobaraensis
29.1
-
 mutant enzyme V30D, at pH 6.0 and 37°C Streptomyces mobaraensis
29.7
-
 mutant enzyme A10S, at pH 6.0 and 37°C Streptomyces mobaraensis
29.8
-
 mutant enzyme M16T/G283S, at pH 6.0 and 37°C Streptomyces mobaraensis
30.1
-
 mutant enzyme Q74L, at pH 6.0 and 37°C Streptomyces mobaraensis
30.2
-
 mutant enzyme V6T, at pH 6.0 and 37°C Streptomyces mobaraensis
30.3
-
 mutant enzyme R238F, at pH 6.0 and 37°C Streptomyces mobaraensis
30.4
-
 mutant enzyme R26L, at pH 6.0 and 37°C Streptomyces mobaraensis
30.5
-
 mutant enzyme D14N, at pH 6.0 and 37°C Streptomyces mobaraensis
30.5
-
 mutant enzyme V30T, at pH 6.0 and 37°C Streptomyces mobaraensis
30.6
-
 mutant enzyme S303A, at pH 6.0 and 37°C Streptomyces mobaraensis
30.6
-
 mutant enzyme S303F, at pH 6.0 and 37°C Streptomyces mobaraensis
30.7
-
 mutant enzyme R238L, at pH 6.0 and 37°C Streptomyces mobaraensis
30.9
-
 mutant enzyme S284T, at pH 6.0 and 37°C Streptomyces mobaraensis
31.4
-
 mutant enzyme T77L, at pH 6.0 and 37°C Streptomyces mobaraensis
31.6
-
 mutant enzyme Y75H, at pH 6.0 and 37°C Streptomyces mobaraensis
32.1
-
 mutant enzyme S299L, at pH 6.0 and 37°C Streptomyces mobaraensis
32.3
-
 mutant enzyme E28D, at pH 6.0 and 37°C Streptomyces mobaraensis
32.3
-
 mutant enzyme P12S, at pH 6.0 and 37°C Streptomyces mobaraensis
32.4
-
 mutant enzyme D3L, at pH 6.0 and 37°C Streptomyces mobaraensis
32.4
-
 mutant enzyme S303T, at pH 6.0 and 37°C Streptomyces mobaraensis
32.7
-
 mutant enzyme E58D, at pH 6.0 and 37°C Streptomyces mobaraensis
33
-
 mutant enzyme H289Y, at pH 6.0 and 37°C Streptomyces mobaraensis
33.4
-
 mutant enzyme R5K, at pH 6.0 and 37°C Streptomyces mobaraensis
33.5
-
 mutant enzyme D3N, at pH 6.0 and 37°C Streptomyces mobaraensis
33.6
-
 mutant enzyme T77A, at pH 6.0 and 37°C Streptomyces mobaraensis
33.8
-
 mutant enzyme V30I, at pH 6.0 and 37°C Streptomyces mobaraensis
34.1
-
 mutant enzyme T77F, at pH 6.0 and 37°C Streptomyces mobaraensis
34.3
-
 mutant enzyme Y34F, at pH 6.0 and 37°C Streptomyces mobaraensis
34.4
-
 mutant enzyme Q74N, at pH 6.0 and 37°C Streptomyces mobaraensis
34.9
-
 mutant enzyme H289F, at pH 6.0 and 37°C Streptomyces mobaraensis
35.5
-
 mutant enzyme R26F, at pH 6.0 and 37°C Streptomyces mobaraensis
35.5
-
 mutant enzyme Y75A, at pH 6.0 and 37°C Streptomyces mobaraensis
35.8
-
 mutant enzyme D3F, at pH 6.0 and 37°C Streptomyces mobaraensis
35.9
-
 mutant enzyme W59F, at pH 6.0 and 37°C Streptomyces mobaraensis
36
-
 mutant enzyme V65I, at pH 6.0 and 37°C Streptomyces mobaraensis
36.6
-
 mutant enzyme T77S, at pH 6.0 and 37°C Streptomyces mobaraensis
37.4
-
 mutant enzyme Q74A, at pH 6.0 and 37°C Streptomyces mobaraensis
41.7
-
 mutant enzyme Y75F, at pH 6.0 and 37°C Streptomyces mobaraensis
42.5
-
 mutant enzyme M16T, at pH 6.0 and 37°C Streptomyces mobaraensis
42.9
-
 mutant enzyme S199A , at pH 6.0 and 37°C Streptomyces mobaraensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-carbobenzoxy-L-glutaminylglycine + NH2OH
-
 Streptomyces mobaraensis hydroxamic acid + ?
-
 ?
N-carbobenzoxy-L-glutaminylglycine + NH2OH
-
 Streptomyces mobaraensis s-8112 hydroxamic acid + ?
-
 ?

Synonyms

Synonyms Comment Organism
MTG
-
 Streptomyces mobaraensis
protein-glutamine gamma-glutamyltransferase
-
 Streptomyces mobaraensis
TGase
-
 Streptomyces mobaraensis