Cloned (Comment) | Organism |
---|---|
cloned into pET21a and overexpressed in Escherichia coli BL21(DE3). The recombinant TGase is formed as inclusion bodies | Streptomyces fradiae |
Protein Variants | Comment | Organism |
---|---|---|
prosuction | expression of enzyme as inclusion bodies in Escherichia coli, and purification using an on-column refolding procedure based on cation SP fast flow chromatography. Protein yield is 53%, and 105 mg from 3.2 g wet weight cells, specific activity is 21 U/mg. Refolded protein demonstrates nearly identical abilities compared with native enzyme | Streptomyces fradiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces fradiae | - |
expression as inclusion bodies in Escherichia coli | - |
Streptomyces fradiae | Q0GYU0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Streptomyces fradiae |
recombinant protein expressed as inclusion bodies in Escherichia coli, application of an on-column refolding procedure based on cation SP fast flow chromatography | Streptomyces fradiae |
Synonyms | Comment | Organism |
---|---|---|
TGase | - |
Streptomyces fradiae |