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Literature summary for 2.3.2.13 extracted from
- Purification and characterization of transglutaminase from tropical tilapia (Oreochromis niloticus) (2005), Food Chem., 93, 651-658.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | slight activation at 5 mM | Oreochromis niloticus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 10 mM, complete inhibition | Oreochromis niloticus | |
| Cu2+ | 10 mM, complete inhibition | Oreochromis niloticus | |
| EDTA | 10 mM, complete inhibition | Oreochromis niloticus | |
| EGTA | 10 mM, complete inhibition | Oreochromis niloticus | |
| iodoacetic acid | 10 mM, complete inhibition | Oreochromis niloticus | |
| K+ | 1 mM, 41% inhibition | Oreochromis niloticus | |
| Li+ | 10 mM, complete inhibition | Oreochromis niloticus | |
| Mg2+ | 1 mM, complete inhibition | Oreochromis niloticus | |
| N-ethylmaleimide | 10 mM, complete inhibition | Oreochromis niloticus | |
| Na+ | 1 mM, 57% inhibition | Oreochromis niloticus | |
| p-chloromercuribenzoate | 10 mM, complete inhibition | Oreochromis niloticus | |
| phenyl methyl sulfonyl fluoride | 10 mM, complete inhibition | Oreochromis niloticus | |
| Zn2+ | 10 mM, complete inhibition | Oreochromis niloticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required, optimal concentration 1.25 mM | Oreochromis niloticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
x * 85000, SDS-PAGE | Oreochromis niloticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oreochromis niloticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oreochromis niloticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 197 | - |
pH 7.5, 37°C | Oreochromis niloticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| monodansylcadaverine + N,N-dimethylcasein | - |
Oreochromis niloticus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 85000, SDS-PAGE | Oreochromis niloticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 50 | - |
Oreochromis niloticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Oreochromis niloticus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Oreochromis niloticus | isoelectric focusing | - |
6.53 |
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