Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 10 mM, approx. 1.8fold activation | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GTP | - |
Glycine max | |
N-ethylmaleimide | 0.1 mM, 60% inhibition | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
spermidine | - |
Glycine max | |
0.069 | - |
spermine | - |
Glycine max | |
0.11 | - |
putrescine | - |
Glycine max |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Ca2+ is not required for activity | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-Sepharose, Blue-Sepharose CL-6B, omega-aminohexyl agarose, alpha-casein agarose | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.16 | - |
- |
Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein-bound gamma-glutamine + alkylamine | - |
Glycine max | protein N5-alkylglutamine + NH3 | - |
? | |
putrescine + N,N'-dimethylcasein | spermine and spermidine can replace putrescine | Glycine max | ? | - |
? | |
spermidine + N,N'-dimethylcasein | - |
Glycine max | ? | - |
? | |
spermine + N,N'-dimethylcasein | - |
Glycine max | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 80000, SDS-PAGE | Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Glycine max |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
GTP | - |
Glycine max |