Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 5 mM; reversible | Physarum polycephalum | |
EGTA | 2 mM, irreversible | Physarum polycephalum | |
N-ethylmaleimide | 0.1 mM | Physarum polycephalum | |
p-chloromercuribenzoate | 0.1 mM | Physarum polycephalum | |
putrescine | 0.1-0.2 mM, substrate inhibition | Physarum polycephalum | |
spermidine | 0.1-0.2 mM, substrate inhibition | Physarum polycephalum | |
spermine | 0.1-0.2 mM, substrate inhibition | Physarum polycephalum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0214 | - |
spermidine | - |
Physarum polycephalum | |
0.0317 | - |
spermine | - |
Physarum polycephalum | |
0.049 | - |
putrescine | - |
Physarum polycephalum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
spheroplast | - |
Physarum polycephalum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | requirement | Physarum polycephalum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39600 | - |
2 * 39600, SDS-PAGE | Physarum polycephalum |
77000 | - |
gel filtration | Physarum polycephalum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein-bound gamma-glutamine + alkylamine | Physarum polycephalum | actin is probably the major endogenous substrate | protein N5-alkylglutamine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Physarum polycephalum | - |
slime mould, strain M3cV | - |
Purification (Comment) | Organism |
---|---|
15% polyethylene glycol, DEAE-cellulos, isoelectric focusing | Physarum polycephalum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.005 | - |
- |
Physarum polycephalum |
Storage Stability | Organism |
---|---|
-20°C, partially purified, 3 years | Physarum polycephalum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein-bound gamma-glutamine + alkylamine | actin is probably the major endogenous substrate | Physarum polycephalum | protein N5-alkylglutamine + NH3 | - |
? | |
putrescine + bovine muscle actin | preferred substrate | Physarum polycephalum | ? | - |
? | |
putrescine + casein | - |
Physarum polycephalum | ? | - |
? | |
putrescine + N,N'-dimethylcasein | spermine can replace putrescine | Physarum polycephalum | ? | - |
? | |
putrescine + N,N'-dimethylcasein | diaminopropane and cadaverine can replace putrescine | Physarum polycephalum | ? | - |
? | |
putrescine + N,N'-dimethylcasein | spermidine can replace putrescine | Physarum polycephalum | ? | - |
? | |
spermidine + N,N'-dimethylcasein | - |
Physarum polycephalum | ? | - |
? | |
spermine + N,N'-dimethylcasein | - |
Physarum polycephalum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 39600, SDS-PAGE | Physarum polycephalum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Physarum polycephalum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 6.1 | Physarum polycephalum |