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Literature summary for 2.3.2.13 extracted from
- Cellular transglutaminase. The particulate-associated transglutaminase from chondrosarcoma and liver: partial purification and characterization (1986), J. Biol. Chem., 261, 8112-8121.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| thrombin | - |
Cavia porcellus | |
| thrombin | - |
Homo sapiens | |
| thrombin | guinea pig epidermal enzyme | Rattus norvegicus | |
| thrombin | particulate enzyme | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | - |
Cavia porcellus |  |
| iodoacetamide | - |
Homo sapiens | |
| iodoacetamide | 86% and 91% inhibition of chondrosarcoma transglutaminase B and C respectively | Rattus norvegicus | |
| additional information | - |
Cavia porcellus | |
| additional information | - |
Homo sapiens | |
| additional information | not inhibited by diisopropylfluorophosphate; not inhibited by PMSF; not inhibited by sulfhydryl-reagents in the absence of Ca2+ | Rattus norvegicus | |
| N-iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylenediamine | 0.01 mM, 91% and 92% inhibition of chondrosarcoma transglutaminases B and C respectively | Rattus norvegicus | |
| p-chloromercuribenzoate | - |
Cavia porcellus | |
| p-chloromercuribenzoate | 21% inhibition of chondrosarcoma transglutaminase B | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Cavia porcellus | |
| additional information | - |
additional information | - |
Homo sapiens | |
| additional information | - |
additional information | kinetic study with various transglutaminases: human factor XIIIa, guinea pig liver TGC, rat chondrosarcoma TGB | Rattus norvegicus | |
| 0.024 | - |
methylamine | transglutaminase C | Cavia porcellus | |
| 0.051 | - |
methylamine | chondrosarcoma transglutaminase B | Rattus norvegicus | |
| 0.061 | - |
methylamine | factor XIIIa | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| particle-bound | - |
Cavia porcellus | - |
- |
| particle-bound | - |
Homo sapiens | - |
- |
| particle-bound | - |
Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | requirement | Cavia porcellus | |
| Ca2+ | requirement | Homo sapiens | |
| Ca2+ | requirement | Rattus norvegicus | |
| Mn2+ | - |
Cavia porcellus | |
| Mn2+ | - |
Homo sapiens | |
| Mn2+ | slight activation | Rattus norvegicus | |
| additional information | - |
Homo sapiens | |
| additional information | not activated by Cu2+ | Rattus norvegicus | |
| Sr2+ | - |
Cavia porcellus | |
| Sr2+ | slight activation | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
- |
Homo sapiens |
| 80000 | - |
liver and chondrosarcoma transglutaminase C, gel filtration | Rattus norvegicus |
| 100000 | - |
liver and chondrosarcoma transglutaminase B | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | chondrosarcoma transglutaminase | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| chondrocyte | malignant, swarm chondrosarcoma | Rattus norvegicus | - |
| liver | - |
Cavia porcellus | - |
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methylamine + succinyl-beta-casein | transglutaminase B | Rattus norvegicus | ? | - |
? | |
| methylamine + succinylated alpha-casein | - |
Cavia porcellus | ? | - |
? | |
| protein-bound gamma-glutamine + alkylamine | - |
Cavia porcellus | protein N5-alkylglutamine + NH3 | - |
? | |
| protein-bound gamma-glutamine + alkylamine | - |
Homo sapiens | protein N5-alkylglutamine + NH3 | - |
? | |
| protein-bound gamma-glutamine + alkylamine | chondrosarcoma transglutaminase B, no activity with type I collagen and fibronectin | Rattus norvegicus | protein N5-alkylglutamine + NH3 | - |
? | |
| protein-bound gamma-glutamine + alkylamine | transglutaminase B: simultaneously gamma-polymer and alpha-polymer formation | Rattus norvegicus | protein N5-alkylglutamine + NH3 | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.32 | - |
methylamine | chondrosarcoma transglutaminase B | Rattus norvegicus | |
| 0.467 | - |
methylamine | transglutaminase C | Cavia porcellus | |
| 1.86 | - |
methylamine | factor XIIIa | Homo sapiens | |
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