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Literature summary for 2.3.2.13 extracted from
- Mechanism of action of guinea pig liver transglutaminase. I. Purification and properties of the enzyme: identification of a functional cysteine essential for activity (1966), J. Biol. Chem., 241, 5518-5525.
General Stability
| General Stability | Organism |
|---|---|
| EDTA, 1-2 mM, stabilizes | Cavia porcellus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) | 1 mol per mol enzyme, 85% inactivation, not reversed by glutathione | Cavia porcellus |  |
| iodoacetamide | 0.1 mM, pH 6.8, in the presence of Ca2+, complete inhibition, irreversible, incorporation of 1 mol carbamidomethyl/mol enzyme, substrate protects | Cavia porcellus | |
| N-ethylmaleimide | 3.6 mol per mol enzyme, 91% inhibition in the presence of Ca2+, not inhibited in the absence of Ca2+, substrate protects | Cavia porcellus | |
| p-chloromercuribenzoate | reversible by glutathione | Cavia porcellus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 66 | - |
carbobenzoxy-L-glutaminylglycine | - |
Cavia porcellus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Cavia porcellus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | requirement | Cavia porcellus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76900 | - |
sedimentation and diffusion | Cavia porcellus |
| 86000 | 94000 | sedimentation equilibrium | Cavia porcellus |
| 90000 | - |
sedimentation equilibrium, meniscus depletion method, iodoacetamide-incorporation studies | Cavia porcellus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
Hartley strain | - |
| Cavia porcellus Hartley | - |
Hartley strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.5 | - |
- |
Cavia porcellus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 5 mM Tris-HCl/2 mM EDTA buffer, pH 7.5, up to 3 months | Cavia porcellus |
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