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Literature summary for 2.3.1.9 extracted from

  • Reddick, J.; Williams, J.
    The mmgA gene from Bacillus subtilis encodes a degradative acetoacetyl-CoA thiolase (2008), Biotechnol. Lett., 30, 1045-1050.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene mmgA, encoded in the mmg operon, DNA and amino acid sequence determination and analysis, overexpression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
acetoacetyl-CoA pH 6.0, 37°C Bacillus subtilis
0.07
-
CoA pH 6.0, 37°C Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
2 * 42000, recombinant His-tagged enzyme, SDS-PAGE Bacillus subtilis
84000
-
about, recombinant His-tagged enzyme, gel filtration Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
gene mmgA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetoacetyl-CoA + CoA the enzyme shows preference for the thiolytic reaction direction Bacillus subtilis 2 acetyl-CoA
-
r

Subunits

Subunits Comment Organism
dimer 2 * 42000, recombinant His-tagged enzyme, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
acetoacetyl-CoA thiolase
-
Bacillus subtilis
MmgA
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
80
-
acetoacetyl-CoA pH 6.0, 37°C Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Bacillus subtilis