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Literature summary for 2.3.1.9 extracted from
- Molecular and catalytic properties of the acetoacetyl-coenzyme A thiolase of Escherichia coli (1976), Arch. Biochem. Biophys., 176, 159-170.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetoacetyl-CoA | substrate inhibition above 0.105 mM | Escherichia coli |  |
| iodoacetamide | 2 mM, complete inactivation after approx. 60 min, 0.3 mM acetoacetyl-CoA or 1.6 mM acetyl-CoA protect | Escherichia coli | |
| N-ethylmaleimide | - |
Escherichia coli | |
| p-chloromercuribenzoate | - |
Escherichia coli | |
| Sodium borohydride | - |
Escherichia coli | |
| sulfhydryl reagents | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.47 | - |
acetyl-CoA | synthesis | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
4 * 42000, SDS-PAGE | Escherichia coli |
| 156000 | - |
sucrose density gradient sedimentation | Escherichia coli |
| 166000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA + acetoacetyl-CoA | Escherichia coli | - |
acetyl-CoA + acetyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| sensitive to borohydride reduction | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1078 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 0.05 M Tris-HCl, pH 7.8, 2 mM dithiothreitol, 1 mM EDTA, 20% glycerol, 6 weeks, 50% loss of activity | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA + acetoacetyl-CoA | - |
Escherichia coli | acetyl-CoA + acetyl-CoA | - |
? | |
| CoA + acetoacetyl-CoA | - |
Escherichia coli | acetyl-CoA + acetyl-CoA | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 42000, SDS-PAGE | Escherichia coli |
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