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Literature summary for 2.3.1.9 extracted from

  • Davis, J.T.; Moore, R.N.; Imperiali, B.; Pratt, A.J.; Kobayashi, K.; Masamune, S.; Sinskey, A.J.; Walsh, C.T.; Fukui, T.; Tomita, K.
    Biosynthetic thiolase from zoogloea ramigera. I. Preliminary characterization and analysis of proton transfer reaction (1987), J. Biol. Chem., 262, 82-89.
    View publication on PubMed

General Stability

General Stability Organism
urea: 2.5 M, 17 h, 50% loss of activity, 5 M, 45 min, 50% loss of activity, 7 M, 1 min, 50% loss of activity Zoogloea ramigera

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide 125fold molar excess, rapid inactivation Zoogloea ramigera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
acetoacetyl-CoA
-
Zoogloea ramigera
0.073
-
acetoacetyl-S-(D-pantetheine) 11-pivalate
-
Zoogloea ramigera
0.074
-
acetoacetyl-S-(11-t-butyldimethylsilyl)pantetheine cosubstrate CoA Zoogloea ramigera
0.12
-
acetoacetyl-S-(11-methoxymethyl)pantetheine
-
Zoogloea ramigera
0.21
-
acetoacetyl-10-bis-demethylpantetheine 11-pivalate
-
Zoogloea ramigera
0.25
-
acetoacetyl-S-homopantetheine 12-pivalate
-
Zoogloea ramigera
0.46
-
acetoacetyl-S-pantetheine
-
Zoogloea ramigera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
162000
-
-
Zoogloea ramigera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + acetoacetyl-CoA Zoogloea ramigera involved in generation of acetoacetyl-CoA for poly-beta-hydroxybutyrate synthesis acetyl-CoA + acetyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Zoogloea ramigera
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-Sepharose, hydroxyapatite, phosphocellulose, Blue Sepharose Zoogloea ramigera

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.16
-
acetoacetyl-CoA synthesis Zoogloea ramigera
412
-
thiolysis of acetoacetyl-CoA Zoogloea ramigera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + acetoacetyl-10-bis-demethylpantetheine 11-pivaloate
-
Zoogloea ramigera acetyl-CoA + acetyl-10-bis-demethylpantetheine 11-pivaloate
-
?
CoA + acetoacetyl-CoA most active in thiolysis Zoogloea ramigera acetyl-CoA + acetyl-CoA
-
r
CoA + acetoacetyl-CoA involved in generation of acetoacetyl-CoA for poly-beta-hydroxybutyrate synthesis Zoogloea ramigera acetyl-CoA + acetyl-CoA
-
?
CoA + acetoacetyl-S-(11-methoxymethyl)pantetheine
-
Zoogloea ramigera acetyl-CoA + acetyl-S-(11-methoxymethyl)pantetheine
-
?
CoA + acetoacetyl-S-(11-t-butyldimethylsilyl)pantetheine
-
Zoogloea ramigera acetyl-CoA + acetyl-S-(11-t-butyldimethylsilyl)pantetheine
-
?
CoA + acetoacetyl-S-(D-pantetheine) 11-pivalate
-
Zoogloea ramigera acetyl-CoA + acetyl-S-(D-pantetheine) 11-pivalate
-
?
CoA + acetoacetyl-S-(L-pantetheine) 11-pivalate
-
Zoogloea ramigera acetyl-CoA + acetyl-S-(L-pantetheine) 11-pivalate
-
?
CoA + acetoacetyl-S-homopantetheine 12-pivalate
-
Zoogloea ramigera acetyl-CoA + acetyl-S-homopantetheine 12-pivalate
-
?
CoA + acetoacetyl-S-pantetheine
-
Zoogloea ramigera acetyl-CoA + acetyl-S-pantetheine
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
174
-
acetoacetyl-S-pantetheine
-
Zoogloea ramigera
177
-
acetoacetyl-S-homopantetheine 12-pivalate
-
Zoogloea ramigera
256
-
acetoacetyl-S-(L-pantetheine) 11-pivalate
-
Zoogloea ramigera
266
-
acetoacetyl-10-bis-demethylpantetheine 11-pivaloate
-
Zoogloea ramigera
353
-
acetoacetyl-S-(11-methoxymethyl)pantetheine
-
Zoogloea ramigera
434
-
acetoacetyl-S-(11-t-butyldimethylsilyl)pantetheine
-
Zoogloea ramigera
465
-
acetoacetyl-CoA
-
Zoogloea ramigera
469
-
acetoacetyl-S-(D-pantetheine) 11-pivalate
-
Zoogloea ramigera