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Literature summary for 2.3.1.9 extracted from

  • Clinkenbeard, K.D.; Sugiyama, T.; Lane, M.D.
    Cytosolic acetoacetyl-CoA thiolase from chicken liver (1975), Methods Enzymol., 35 B, 167-173.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
CoA higher than 0.03 mM, inhibition in cleavage direction Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0064
-
CoA
-
Gallus gallus
0.0385
-
acetoacetyl-CoA
-
Gallus gallus
0.27
-
acetyl-CoA
-
Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Gallus gallus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
4 * 41000, SDS-PAGE Gallus gallus
169000
-
sedimentation equilibrium Gallus gallus
188000
-
gel filtration Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + acetoacetyl-CoA Gallus gallus
-
acetyl-CoA + acetyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
liver enzyme, ammonium sulfate, calcium phosphate gel, phosphocellulose column, DEAE-cellulose, hydroxylapatite, Sephadex G-200 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
assay method Gallus gallus
203
-
-
Gallus gallus

Storage Stability

Storage Stability Organism
-90°C, 12 months Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + acetoacetyl-CoA
-
Gallus gallus acetyl-CoA + acetyl-CoA
-
?
CoA + acetoacetyl-CoA
-
Gallus gallus acetyl-CoA + acetyl-CoA
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 41000, SDS-PAGE Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Gallus gallus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
29.5
-
CoA presence of Mg2+ Gallus gallus
900
-
thiolytic cleavage
-
Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
cleavage of acetoacetyl-CoA, 85% of maximal activity between pH 8.0 and pH 9.0 Gallus gallus