Activating Compound | Comment | Organism | Structure |
---|---|---|---|
forskolin | decreases KM-value for tryptamine by 32% | Rattus norvegicus | |
phorbol 12-myristate 13-acetate | decreases KM-value for tryptamine by 44% | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
S192V | decrease in Km-value obtained by treatment with phorbol 12-myristate 13-acetate or forskolin is similar to the decrease observed in wild-type cells | Rattus norvegicus |
T127V | decrease in Km-value obtained by treatment with phorbol 12-myristate 13-acetate or forskolin is similar to the decrease observed in wild-type cells | Rattus norvegicus |
T29V | mutant enzyme shows an increase in Km-value obtained by treatment with phorbol 12-myristate 13-acetate or forskolin, decrease in Km-value is observed in wild-type cells | Rattus norvegicus |
T29V/S203G | mutant enzyme shows an increase in Km-value obtained by treatment with phorbol 12-myristate 13-acetate or forskolin, decrease in Km-value is observed in wild-type cells | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.291 | - |
tryptamine | pH 6.8, 37°C, mutant enzyme S192V | Rattus norvegicus | |
0.317 | - |
tryptamine | pH 6.8, 37°C, mutant enzyme T29V/S203G | Rattus norvegicus | |
0.344 | - |
tryptamine | pH 6.8, 37°C, mutant enzyme T127V | Rattus norvegicus | |
0.361 | - |
tryptamine | pH 6.8, 37°C, wild-type enzyme | Rattus norvegicus | |
0.413 | - |
tryptamine | pH 6.8, 37°C, mutant enzyme T29V | Rattus norvegicus | |
0.417 | - |
tryptamine | pH 6.8, 37°C, mutant enzyme S203G | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylated at Thr29 by protein kinase C activation through the alpha1-adrenergic receptor in rat pineal glands. Phosphorylation may contribute to the stability and activity of the enzyme | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pineal gland | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + tryptamine | - |
Rattus norvegicus | CoA + N-acetyltryptamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AANAT | - |
Rattus norvegicus |
serotonin N-acetyltransferase | - |
Rattus norvegicus |