Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-O-methylmannose | 0.1 mM, lowers Km 4fold, activation by relieving product inhibition by binding long-chain acyl-CoA | Mycolicibacterium phlei | |
6-O-methylglucose | 0.1 mM, lowers Km 4fold, activation by relieving product inhibition by binding long-chain acyl-CoA | Mycolicibacterium phlei |
General Stability | Organism |
---|---|
low ionic strength, 0.005 M, leads to dissociation into subunits, partially reactivated by increasing the ionic strength to 0.5 M | Mycolicibacterium phlei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0096 | - |
malonyl-CoA | in presence of polysaccharide | Mycolicibacterium phlei | |
0.04 | - |
malonyl-CoA | - |
Mycolicibacterium phlei | |
0.09 | - |
acetyl-CoA | in presence of polysaccharide | Mycolicibacterium phlei | |
0.8 | - |
acetyl-CoA | - |
Mycolicibacterium phlei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
1390000 | - |
sedimentation velocity data | Mycolicibacterium phlei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium phlei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
homogeneity | Mycolicibacterium phlei |
Renatured (Comment) | Organism |
---|---|
low concentration of phosphate buffer causes dissociation into inactive species, reaggregation and reactivation can be partially achieved by dialysis against 0.5 M phosphate buffer | Mycolicibacterium phlei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.375 | - |
- |
Mycolicibacterium phlei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 7 malonyl-CoA + 7 NADH + 7 NADPH + 14 H+ | primers instead of acetyl-CoA: propionyl-CoA, butyryl-CoA or hexanoyl-CoA | Mycolicibacterium phlei | palmitoyl-CoA + 7 CoA + 7 CO2 + 7 NAD+ + 7 NADP+ + 7 H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Mycolicibacterium phlei | |
NADPH | requirement | Mycolicibacterium phlei |