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Literature summary for 2.3.1.85 extracted from

  • McCarthy, A.D.; Hardie, D.G.
    The multifunctional polypeptide chains of rabbit-mammary fatty-acid synthase. Stoichiometry of active sites and active-site mapping using limited proteolysis (1983), Eur. J. Biochem., 130, 185-193.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,3-Dibromo-2-propanone acetyl-CoA, not malonyl-CoA, protects against inactivation, both protect against cross-linking of the subunits Oryctolagus cuniculus
chloroacetyl-CoA
-
Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
molecular weight of fragments after proteolytic cleavage Oryctolagus cuniculus
250000
-
2 * 250000, SDS-PAGE Oryctolagus cuniculus

Organic Solvent Stability

Organic Solvent Comment Organism
acetonitrile up to 10% v/v, stable to Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
female
-

Purification (Commentary)

Purification (Comment) Organism
-
Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
mammary gland lactating Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+
-
Oryctolagus cuniculus palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
-
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Subunits

Subunits Comment Organism
dimer 2 * 250000, SDS-PAGE Oryctolagus cuniculus
More alpha2-dimer of multifunctional subunits: each subunit contains up to seven active centres required for palmitate synthesis Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
4'-phosphopantetheine location of active site Oryctolagus cuniculus
4'-phosphopantetheine present in substoichiometric amounts Oryctolagus cuniculus