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Literature summary for 2.3.1.85 extracted from

  • Rangan, V.S.; Smith, S.
    Alteration of the substrate specificity of the malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase domain of the multifunctional fatty acid synthase by mutation of a single arginine residue (1997), J. Biol. Chem., 272, 11975-11978.
    View publication on PubMed
Search for more literature for 2.3.1.85

Cloned(Commentary)

Cloned (Comment) Organism
transacyclase domain Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
R606A reduced malonyl/acetyltransferase activity, increased transacylase activity, 16000fold increased selectivity for acetyl-CoA, 8.5fold increase of Km for malonyl-CoA Rattus norvegicus
R606K reduced malonyl/acetyltransferase activity, increased transacylase activity, 16fold increased selectivity for acetyl-CoA Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 malonyl-CoA R606K mutant Rattus norvegicus
0.0018
-
 acetyl-CoA R606A mutant Rattus norvegicus
0.0019
-
 malonyl-CoA wild-type enzyme Rattus norvegicus
0.0029
-
 acetyl-CoA R606K mutant Rattus norvegicus
0.0039
-
 acetyl-CoA wild-type enzyme Rattus norvegicus
0.0162
-
 malonyl-CoA R606A mutant Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 wild-type and mutant
-

Purification (Commentary)

Purification (Comment) Organism
95% pure, transacyclase domain Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.015
-
 for malonyl-CoA, R606A mutant Rattus norvegicus
0.154
-
 for malonyl-CoA, R606K mutant Rattus norvegicus
1.61
-
 for malonyl-CoA, wild-type enzyme Rattus norvegicus
2.68
-
 for acetyl-CoA, wild-type enzyme Rattus norvegicus
4.53
-
 for acetyl-CoA, R606K mutant Rattus norvegicus
17.8
-
 for acetyl-CoA, R606A mutant Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0002
-
 malonyl-CoA R606K mutant Rattus norvegicus
0.00167
-
 malonyl-CoA R606A mutant Rattus norvegicus
0.02
-
 malonyl-CoA wild-type enzyme Rattus norvegicus
0.0317
-
 acetyl-CoA wild-type enzyme Rattus norvegicus
0.0567
-
 acetyl-CoA R606K mutant Rattus norvegicus
0.237
-
 acetyl-CoA R606A mutant Rattus norvegicus