Activating Compound | Comment | Organism | Structure |
---|---|---|---|
reduced ferredoxin | reduced flavodoxin is te better cofactor | Escherichia coli | |
reduced flavodoxin | a better electron donor than reduced ferredoxin | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
gene pflB, the enzyme and its activating enzyme from Escherichia coli are expressed in a Saccharomyces cerevisiae strain IMI076 lacking pyruvate decarboxylase and having a reduced glucose uptake rate due to a mutation in the transcriptional regulator Mth1. Enzyme PFL is expressed with two different electron donors, reduced ferredoxin or reduced flavodoxin, respectively. The coexpression of either of these electron donors has a positive effect on growth under aerobic conditions, indicating increased activity of PFL. | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P09373 | gene pflB | - |
Synonyms | Comment | Organism |
---|---|---|
PFL | - |
Escherichia coli |
pyruvate formate lyase | - |
Escherichia coli |
Organism | Comment | Expression |
---|---|---|
Escherichia coli | coexpression of either of the electron donors reduced ferredoxin or reduced flavodoxin has a positive effect on growth under aerobic conditions, indicating increased activity of PFL. with a higher final biomass concentration and a significant increase in transcription of formate dehydrogenases | up |