Cloned (Comment) | Organism |
---|---|
gene deletion is transduced via Bacteriophage P1 | Escherichia coli str. K-12 substr. W3110 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant strain YK167 lacking pyruvate formate-lyase shows almost 50% increase in glucose consumption and flux with higher flux to lactate and lower ATP-yield, double mutant for pyruvate formate-lyase and lactate dehydrogenase (strain SE2382) shows 50% lower glucose flux and ATP yield than strain YK167 and results in reduced cell mass growth | Escherichia coli str. K-12 substr. W3110 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + pyruvate | Escherichia coli str. K-12 substr. W3110 | anaerobic growth under nitrogen, 37°C, pH 7.0, extract enzyme reactions measured at room temperature | acetyl-CoA + formate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli str. K-12 substr. W3110 | - |
wild-type and mutant strains, anaerobic growth conditions | - |
Purification (Comment) | Organism |
---|---|
centrifugation, washing in 50 mM potassium phosphate buffer, pH 7.5, resuspension, French press disruption, centrifugation of crude extract and of resulting supernatant | Escherichia coli str. K-12 substr. W3110 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + pyruvate | anaerobic growth under nitrogen, 37°C, pH 7.0, extract enzyme reactions measured at room temperature | Escherichia coli str. K-12 substr. W3110 | acetyl-CoA + formate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PFL | - |
Escherichia coli str. K-12 substr. W3110 |
pyruvate formate-lyase | - |
Escherichia coli str. K-12 substr. W3110 |
General Information | Comment | Organism |
---|---|---|
metabolism | anaerobic fermentation pathway yielding 3 ATP per glucose, pyruvate channeling to fermentation product, lactate dehydrogenase and pyruvate dehydrogenase are alternative pyruvate channeling enzymes during fermentation | Escherichia coli str. K-12 substr. W3110 |