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Literature summary for 2.3.1.54 extracted from
- Inactivation of E. coli pyruvate formate-lyase: role of AdhE and small molecules (2007), Arch. Biochem. Biophys., 459, 1-9.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of the His6-tagged enzyme as single enzyme or as AdhE-Pfl in strain BL21(DE3) | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | rapid, reversible inactivation, deactivation is a non-destructive transfer of an H atom equivalent to quench the glycyl radical | Escherichia coli |  |
| DTT | reversible inactivation, deactivation is a non-destructive transfer of an H atom equivalent to quench the glycyl radical | Escherichia coli | |
| additional information | the putative pyruvate formate-lyase deactivase, as activity of AdhE together with an alcohol dehydrogenase and an acetaldehyde-CoA dehydrogenase activities, is not active on the enzyme PFL, it has no PFL deactivating activity | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA + pyruvate | Escherichia coli | - |
acetyl-CoA + formate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene pfl | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from strain BL21(DE3) by gel filtration and anion exchange chromatography, recombinant His6-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography as single enzyme or as AdhE-Pfl | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acetyl-CoA + formate = CoA + pyruvate | the reaction mechanism proceeds via generation of a catalytically essential glycyl radical at Gly734 | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA + pyruvate | - |
Escherichia coli | acetyl-CoA + formate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PFL | - |
Escherichia coli |
| pyruvate formate-lyase | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Escherichia coli |
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Release 2023.1 (January 2023)
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