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Literature summary for 2.3.1.50 extracted from

  • Taouji, S.; Higa, A.; Delom, F.; Palcy, S.; Mahon, F.X.; Pasquet, J.M.; Bosse, R.; Segui, B.; Chevet, E.
    Phosphorylation of serine palmitoyltransferase long chain-1 (SPTLC1) on tyrosine 164 inhibits its activity and promotes cell survival (2013), J. Biol. Chem., 288, 17190-17201.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.50

Protein Variants

Protein Variants Comment Organism
Y164F site-directed mutagenesis, the mutant shows increased serine palmitoyltransferase activity compared to the wild-type enzyme. The Y164F mutation also promotes the remodeling of cellular sphingolipid content, thereby sensitizing K562 cells to apoptosis. the Y164F mutation affects SPTLC1 subcellular localization, induction of apoptosis, and sell sensitivity to imatinib Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Homo sapiens 5783
-
endoplasmic reticulum
-
 Canis lupus familiaris 5783
-
microsome
-
 Homo sapiens
-
-
microsome rough endoplasmic reticulum microsomes Canis lupus familiaris
-
-
additional information ABL-mediated phosphorylation of enzyme SPTLC1 affects its endoplasmic reticulum localization Homo sapiens
-
-
additional information ABL-mediated phosphorylation of enzyme SPTLC1 affects its endoplasmic reticulum localization Canis lupus familiaris
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Homo sapiens
Mn2+ required Canis lupus familiaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
palmitoyl-CoA + L-serine Homo sapiens
-
 CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine Canis lupus familiaris
-
 CoA + 3-dehydro-D-sphinganine + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris E2RKV3
-
-
Homo sapiens O15269 subunit hLCB1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein phosphorylation of serine palmitoyltransferase long chain-1 (SPTLC1) on tyrosine 164 by the fusion kinase ABL inhibits the SPTLC1 enzyme activity. ABL-mediated phosphorylation of SPTLC1 affects its endoplasmic reticulum localization Canis lupus familiaris
phosphoprotein phosphorylation of serine palmitoyltransferase long chain-1 (SPTLC1) on tyrosine 164 by the fusion kinase BCR-ABL inhibits the SPTLC1 enzyme activity. Inhibition of BCR-ABL kinase using either imatinib or shRNA-mediated silencing leads to the activation of SPTLC1 and to increased apoptosis in both K562 and LAMA-84 cells. ABL-mediated phosphorylation of SPTLC1 affects its endoplasmic reticulum localization Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
K-562 cell
-
 Homo sapiens
-
LAMA-84 cell
-
 Homo sapiens
-
pancreas
-
 Canis lupus familiaris
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + L-serine
-
 Homo sapiens CoA + 3-dehydro-D-sphinganine + CO2
-
 ?
palmitoyl-CoA + L-serine
-
 Canis lupus familiaris CoA + 3-dehydro-D-sphinganine + CO2
-
 ?

Synonyms

Synonyms Comment Organism
SPT
-
 Homo sapiens
SPT
-
 Canis lupus familiaris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens
37
-
 assay at Canis lupus familiaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens
8
-
 assay at Canis lupus familiaris

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate required Homo sapiens
pyridoxal 5'-phosphate required Canis lupus familiaris

Expression

Organism Comment Expression
Homo sapiens imatinib does not affect SPTLC1 expression additional information
Canis lupus familiaris imatinib does not affect SPTLC1 expression additional information

General Information

General Information Comment Organism
malfunction enzyme inhibition promotes cell survival Homo sapiens
malfunction enzyme inhibition promotes cell survival Canis lupus familiaris
metabolism serine palmitoyltransferase long chain-1 (SPTLC1) is the first enzyme of sphingolipid biosynthesis Homo sapiens
physiological function phosphorylation of serine palmitoyltransferase long chain-1 (SPTLC1) on tyrosine 164 by the fusion kinase BCR-ABL inhibits the SPTLC1 enzyme activity. Inhibition of BCR-ABL kinase using either imatinib or shRNA-mediated silencing leads to the activation of SPTLC1 and to increased apoptosis in both K562 and LAMA-84 cells, a mechanistic explanation for imatinib-mediated cell death Homo sapiens