Literature summary for 2.3.1.50 extracted from

Gan, Y.; Zhang, L.; Zhang, Z.; Dong, S.; Li, J.; Wang, Y.; Zheng, X.
The LCB2 subunit of the sphingolip biosynthesis enzyme serine palmitoyltransferase can function as an attenuator of the hypersensitive response and Bax-induced cell death (2009), New Phytol., 181, 127-146.

Cloned(Commentary)

Cloned (Comment)	Organism
LCB2 cloning by ESTs, DNA and amino acid sequence determination and analysis, LCB2 expression analysis and phylogenetic analysis, overexpression of subunit LCB2 in Saccharomyces cerevisiae strain W303, transient overexpression in Nicotiana tabacum leaves via Agrobacterium tumefaciens strain GV2260-mediated transfection. BcLCB2 encodes a bona fide LCB2 subunit and the SPT activity in yeast results from co-expression of the BcLCB1 and BcLCB2 subunits	Brassica rapa subsp. oleifera
subunits LCB1 and LCB2 expression analysis	Arabidopsis thaliana

Cloned (Comment)

Organism

LCB2 cloning by ESTs, DNA and amino acid sequence determination and analysis, LCB2 expression analysis and phylogenetic analysis, overexpression of subunit LCB2 in Saccharomyces cerevisiae strain W303, transient overexpression in Nicotiana tabacum leaves via Agrobacterium tumefaciens strain GV2260-mediated transfection. BcLCB2 encodes a bona fide LCB2 subunit and the SPT activity in yeast results from co-expression of the BcLCB1 and BcLCB2 subunits

Brassica rapa subsp. oleifera

subunits LCB1 and LCB2 expression analysis

Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
K311E	construction of mutant BcLCB2DELTAK311E that shows reduced activity compared to the wild-type enzyme	Brassica rapa subsp. oleifera
additional information	construction of an inactive pyridoxal 5'-phosphate binding site deletion mutant BcLCB2DELTA307-314. Overexpression of BcLCB2 in Nicotiana tabacum cv. Xanthi leaves suppresses the hypersensitive cell death initiated by elicitors and PB90-triggered H2O2 accumulation, while NbLCB2 silencing in Nicotiana benthamiana enhances elicitor-triggered hypersensitive cell death. BcLCB2 overexpression suppresses Bax- and oxidant stress-triggered yeast cell death. Reactive oxygen species accumulation induced by Bax is compromised in BcLCB2-overexpressing Saccharomyces cerevisiae strain W303 cells, detailed overview	Brassica rapa subsp. oleifera

Inhibitors

Inhibitors	Comment	Organism	Structure
myriocin	a potent inhibitor of SPT, that has no effect on Bax-induced programmed cell death	Brassica rapa subsp. oleifera

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Arabidopsis thaliana	-	-
microsome	-	Brassica rapa subsp. oleifera	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Brassica rapa subsp. oleifera	the LCB2 subunit of the sphingolipid biosynthesis enzyme SPT can function as an attenuator of the hypersensitive response and Bax-induced cell death, overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q94IB8	LCB1	-
Arabidopsis thaliana	Q9LSZ9	LCB2	-
Brassica rapa subsp. oleifera	B5LED9	LCB2; ssp. chinensis	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
flower	expression of LCB1	Arabidopsis thaliana	-
flower	expression of LCB2	Arabidopsis thaliana	-
flower	expression of LCB2	Brassica rapa subsp. oleifera	-
leaf	young, expression of LCB1	Arabidopsis thaliana	-
leaf	young, expression of LCB2	Arabidopsis thaliana	-
leaf	young, expression of LCB2	Brassica rapa subsp. oleifera	-
additional information	AtLCB1 is expressed ubiquitously in all organs of Arabidopsis thaliana examined, but the transcript level is higher in flowers and stems than in leaves and roots	Arabidopsis thaliana	-
additional information	AtLCB2 is expressed ubiquitously in all organs of Arabidopsis thaliana examined, but the transcript level is higher in flowers and stems than in leaves and roots	Arabidopsis thaliana	-
additional information	BcLCB2 expression is detected by RT-PCR in all nonheading Chinese cabbage organs	Brassica rapa subsp. oleifera	-
root	expression of LCB1	Arabidopsis thaliana	-
root	expression of LCB2	Arabidopsis thaliana	-
root	expression of LCB2	Brassica rapa subsp. oleifera	-
stem	expression of LCB1	Arabidopsis thaliana	-
stem	expression of LCB2	Arabidopsis thaliana	-
stem	expression of LCB2	Brassica rapa subsp. oleifera	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the LCB2 subunit of the sphingolipid biosynthesis enzyme SPT can function as an attenuator of the hypersensitive response and Bax-induced cell death, overview	Brassica rapa subsp. oleifera	?	-	?

Subunits

Subunits	Comment	Organism
dimer	the enzyme is formed by two subunits, LCB1 and LCB2, i.e. long-chain base 1 and 2	Arabidopsis thaliana
dimer	the enzyme is formed by two subunits, LCB1 and LCB2, i.e. long-chain base 1 and 2	Brassica rapa subsp. oleifera

Synonyms

Synonyms	Comment	Organism
serine palmitoyltransferase	-	Arabidopsis thaliana
serine palmitoyltransferase	-	Brassica rapa subsp. oleifera
SPT	-	Arabidopsis thaliana
SPT	-	Brassica rapa subsp. oleifera

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	subunit LCB2 possesses a binding site	Brassica rapa subsp. oleifera

Expression

Organism	Comment	Expression
Brassica rapa subsp. oleifera	LCB2 is upregulated during hypersensitive cell death nduced by the Phytophthora boehmeriae elicitor PB90	up

General Information

General Information	Comment	Organism
physiological function	the LCB2 subunit of the sphingolipid biosynthesis enzyme SPT can function as an attenuator of the hypersensitive response and Bax-induced cell death, not involved in the dominant-negative effect that results from BcLCB2 overexpression, overview	Brassica rapa subsp. oleifera