Application | Comment | Organism |
---|---|---|
molecular biology | the catalytically inactive mutant Y891F is useful for stable binding and purification of unacetylated histone H4 protein | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression of GST-tagged enzyme in BSC40 cells via VTF-7 vaccinia virus transfection vector | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
Y891F | site-directed mutagenesis, catalytically inactive mutant, stable binding of unacetylated histone H4, useful for purification of the histone protein | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleoplasm | - |
Mus musculus | 5654 | - |
nucleosome | - |
Mus musculus | 786 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not bind Zn2+ | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
histone H4 + acetyl-CoA | Mus musculus | - |
acetyl-histone H4 + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from BSC40 cells by glutathione affinity chromatography | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
histone H4 + acetyl-CoA | - |
Mus musculus | acetyl-histone H4 + CoA | - |
? | |
histone H4 + acetyl-CoA | acetylation at Lys8, NCOAT has the ability to directly associate with both an acetylated and unacetylated histone H4 tail in vitro without tequiring acetyl-lysine contacts, binding and interaction mechanism, overview | Mus musculus | acetyl-histone H4 + CoA | - |
? | |
additional information | the nuclear cytoplasmic O-GlcNAcase and acetyltransferase, NCOAT, is a bifunctional enzyme with both glycoside hydrolase and alkyltransferase activity and contains a zinc finger-like motif responsible for substrate recognition, via making contacts with the histone tails within nucleosomes, essential for activity, overview | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | NCOAT contains a motif similar to the histone binding domain in the MYST family of HATs, a zinc finger-like motif responsible for substrate recognition | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
HAT | - |
Mus musculus |
More | the enzyme belongs to the MYST family of histone acetyltransferases | Mus musculus |
NCOAT | - |
Mus musculus |
nuclear cytoplasmicO-GlcNAcase and acetyltransferase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Mus musculus |