Any feedback?
Literature summary for 2.3.1.48 extracted from
- Application of a fluorescent histone acetyltransferase assay to probe the substrate specificity of the human p300/CBP-associated factor (2000), Anal. Biochem., 287, 319-328.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of enzyme domain PCAF amino acid residues 493-658 in Escherichia coli | Homo sapiens |
| overexpression of enzyme domain, tGcn5 consists of amino acid residues 48-210 | Tetrahymena thermophila |
| overexpression of enzyme domain, yGcn5 consists of amino acid residues 99-262 | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.75 | - |
histone H3-peptide p27 | PCAF catalytic domain | Homo sapiens | |
| 1.12 | - |
histone H3-peptide p19 | PCAF catalytic domain | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Tetrahymena thermophila |
| additional information | - |
three-dimensional structure of the ternary complex of tGcn5 domain | Homo sapiens |
| additional information | - |
three-dimensional structure of the ternary complex of tGcn5 domain | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
GCN5 | - |
| Homo sapiens | - |
PCAF protein | - |
| Homo sapiens GCN5 | - |
GCN5 | - |
| Saccharomyces cerevisiae | - |
gene Gcn5 | - |
| Tetrahymena thermophila | - |
GCN5 | - |
| Tetrahymena thermophila GCN5 | - |
GCN5 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant catalytic domain of Gcn5 protein | Saccharomyces cerevisiae |
| recombinant enzyme domains of PCAF and Gcn5 | Homo sapiens |
| tGcn5 domain, recombinant from E. coli | Tetrahymena thermophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + histone | - |
Homo sapiens | CoA + acetylhistone | - |
ir |  |
| acetyl-CoA + histone | - |
Saccharomyces cerevisiae | CoA + acetylhistone | - |
? | |
| acetyl-CoA + histone | - |
Tetrahymena thermophila | CoA + acetylhistone | - |
? | |
| acetyl-CoA + histone | - |
Homo sapiens GCN5 | CoA + acetylhistone | - |
ir | |
| acetyl-CoA + histone | - |
Tetrahymena thermophila GCN5 | CoA + acetylhistone | - |
? | |
| acetyl-CoA + histone H3 | - |
Saccharomyces cerevisiae | CoA + acetylhistone H3 | - |
? | |
| acetyl-CoA + histone H3 | PCAF protein | Homo sapiens | CoA + acetylhistone H3 | - |
? | |
| acetyl-CoA + histone H3 | PCAF protein | Homo sapiens GCN5 | CoA + acetylhistone H3 | - |
? | |
| acetyl-CoA + histone H3 peptide | peptides H3p19, H3p27, H3p11 are substrates for the catalytic domain of Gcn5 and PCAF | Homo sapiens | CoA + acetylhistone H3 peptide | - |
? | |
| acetyl-CoA + histone H3 peptide | peptides H3p19, H3p27, H3p11 are substrates for the catalytic domain of Gcn5 and PCAF | Saccharomyces cerevisiae | CoA + acetylhistone H3 peptide | - |
? | |
| acetyl-CoA + histone H3 peptide | peptides H3p19, H3p27, H3p11 are substrates for the catalytic domain of Gcn5 and PCAF | Tetrahymena thermophila | CoA + acetylhistone H3 peptide | - |
? | |
| acetyl-CoA + histone H3 peptide | peptide H3p20 | Homo sapiens | CoA + acetylhistone H3 peptide | - |
? | |
| acetyl-CoA + histone H3 peptide | peptide H3p20 | Saccharomyces cerevisiae | CoA + acetylhistone H3 peptide | - |
? | |
| acetyl-CoA + histone H3 peptide | peptide H3p20 | Tetrahymena thermophila | CoA + acetylhistone H3 peptide | - |
? | |
| acetyl-CoA + histone H3 peptide | peptides H3p19, H3p27, H3p11 are substrates for the catalytic domain of Gcn5 and PCAF | Homo sapiens GCN5 | CoA + acetylhistone H3 peptide | - |
? | |
| acetyl-CoA + histone H3 peptide | peptide H3p20 | Homo sapiens GCN5 | CoA + acetylhistone H3 peptide | - |
? | |
| acetyl-CoA + histone H3 peptide | peptides H3p19, H3p27, H3p11 are substrates for the catalytic domain of Gcn5 and PCAF | Tetrahymena thermophila GCN5 | CoA + acetylhistone H3 peptide | - |
? | |
| acetyl-CoA + histone H3 peptide | peptide H3p20 | Tetrahymena thermophila GCN5 | CoA + acetylhistone H3 peptide | - |
? | |
| acetyl-CoA + histone H3-peptide p19 | - |
Homo sapiens | CoA + acetylhistone H3-peptide p19 | - |
? | |
| acetyl-CoA + histone H3-peptide p19 | - |
Homo sapiens | CoA + acetylhistone H3 -peptide p19 | - |
? | |
| acetyl-CoA + histone H3-peptide p27 | - |
Homo sapiens | CoA + acetylhistone H3 -peptide p27 | - |
? | |
| acetyl-CoA + histone H4 | - |
Saccharomyces cerevisiae | CoA + acetylhistone H4 | - |
? | |
| acetyl-CoA + histone H4 | - |
Tetrahymena thermophila | CoA + acetylhistone H4 | - |
? | |
| acetyl-CoA + histone H4 | PCAF protein | Homo sapiens | CoA + acetylhistone H4 | - |
? | |
| acetyl-CoA + histone H4 | PCAF protein | Homo sapiens GCN5 | CoA + acetylhistone H4 | - |
? | |
| acetyl-CoA + histone H4 | - |
Tetrahymena thermophila GCN5 | CoA + acetylhistone H4 | - |
? | |
| acetyl-CoA + protein p53 | peptide of p53 is a substrate for PCAF catalytic domain | Homo sapiens | CoA + acetylprotein p53 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HAT | - |
Homo sapiens |
| HAT | - |
Saccharomyces cerevisiae |
| HAT | - |
Tetrahymena thermophila |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.131 | - |
histone H3-peptide p19 | PCAF catalytic domain | Homo sapiens | |
| 0.17 | - |
histone H3-peptide p27 | PCAF catalytic domain | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
