Cloned (Comment) | Organism |
---|---|
expression of Gcn5 protein catalytic domain in Escherichia coli BL21 (DE3) | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetylated histone H3 peptide | noncompetitive versus acetyl-CoA and histone H3 | Saccharomyces cerevisiae | |
CoA | competitive versus acetyl-CoA, Gnc5 protein | Saccharomyces cerevisiae | |
desulfo-coenzyme A | dead-end inhibitor, competitive versus acetyl-CoA, Gcn5 protein | Saccharomyces cerevisiae | |
etheno-CoA | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
acetyl-CoA | Gcn5 protein | Saccharomyces cerevisiae | |
0.49 | - |
histone H3-peptide | Gcn5 protein | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | 7fold decrease in activity between 0.15 and 1 M | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
gene Gcn5 | - |
Purification (Comment) | Organism |
---|---|
recombinant catalytic domain of Gcn5 protein | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine | ternary complexed mechanism for PCAF and Gnc5 | Saccharomyces cerevisiae | |
acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine | PCAF and Gcn5 protein, fully ordered Bi-Bi kinetic mechanism with acetyl-CoA binding before histone H3 | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + histone | - |
Saccharomyces cerevisiae | CoA + acetylhistone | - |
r | |
acetyl-CoA + histone H3 | - |
Saccharomyces cerevisiae | CoA + acetylhistone H3 | - |
? | |
acetyl-CoA + histone H3 peptide | Lys14 of histone H3 and a peptide containing Lys14 thereof are the preferred substrates for Gnc5 and PCAF protein, as well as Gnc5 and PCAF catalytic domain | Saccharomyces cerevisiae | CoA + acetylhistone H3 peptide | - |
? | |
acetyl-CoA + histone H3-peptide | - |
Saccharomyces cerevisiae | CoA + acetylhistone H3-peptide | - |
? | |
acetyl-CoA + histone H3-peptide | - |
Saccharomyces cerevisiae | CoA + acetylhistone H3 -peptide | - |
? | |
additional information | Gcn5 and PCAF protein are transcription cofactors | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HAT | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0283 | - |
acetyl-CoA | Gcn5 protein | Saccharomyces cerevisiae | |
0.0283 | - |
histone H3-peptide | Gcn5 protein | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Saccharomyces cerevisiae |